Projections of diencephalic dopamine neurons into the spinal cord in mice

The aim of this study is to investigate the pathway of diencephalic dopaminergic (DA) neuronal innervating into the spinal cord in mice, the pathway is postulated relevant to clinical restless legs syndrome (RLS). Tyrosine hydroxylase (TH) immunohistochemstry was used to identify the DA neuron. The fluorescent tracer Fluoro-Gold (FG) was sterotaxically injected into the T₁₀–L₅ spinal cord of CBL57 mice (n=20) seven days before the animals were sacrificed. The diencephalic sections were stained with TH antibody and the FG tracer present in the diencephalic DA neurons were examined under fluoresce microscope. The average number of total DA neurons per side in A₁₁, A₁₂, A₁₃ and A₁₄ was 66±8, 221±12, 350±17 and 254±21 respectively. After being injected into the spinal cord, FG reached the DA neurons within the A₁₀ and A₁₁ groups, but didn’t target to any other DA neuron groups including the A₈ and A₉ groups in substantia nigra (SN). The diencephalic A₁₁ DA neurons possessed long axons extending over several segments and possibly traversing the entire length of the spinal cord. It is the first time to report A₁₀ and A₁₁ DA neuron projections into the spinal cord in mice. Grant sponsor: NINDS (40370 and 043567); Glaxo SmithKline Pharmaceuticals