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| HPLC-DAD法同时测定玄参炮制前后8个有效成分的含量(英文) | |
| 引用本文： | 曹岗,丛晓东,蔡皓,李晓萌,季金玉,张云,蔡宝昌. HPLC-DAD法同时测定玄参炮制前后8个有效成分的含量(英文)[J]. 中国天然药物, 2012, 0(3): 213-217 |
| 作者姓名： | 曹岗 丛晓东 蔡皓 李晓萌 季金玉 张云 蔡宝昌 |
| 作者单位： | [1]浙江中医药大学中药炮制技术研究中心,杭州310053 [2]南京中医药大学药学院,南京210046 [3]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,南京210029 [4]南京海昌中药集团有限公司,南京210061 |
| 基金项目： | supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions,PAPD （No.ysxk-2010）;the Open Project Program of National First-Class Key Discipline for Science of Chinese Materia Medica,Nanjing University of Chinese Medicine （No.2011ZYX2- 006）;the research fund of Ministry of health of the people’s republic of China （No. WKJ2010-2-019）;Zhejiang Province Chinese medicine research program （No.2008ZA002）;the fund of Zhejiang modernization of traditional Chinese medicine item （No.[2008]436）;Science Foundation of Zhejiang Chinese Medical University （No.7211093）~~ |
| 摘 要： | 目的:同时测定不同来源玄参生品与炮制品中8个主要成分的含量;方法:采用AgilentZorbaxExtendC18色谱柱(250mm×4.6mm,5μm),二极管阵列检测器;以0.3%磷酸水溶液-乙腈为流动相,梯度洗脱,流速为1.0mL·min-1,检测波长为280nm和210nm,柱温为30°C。结果:8个主要成分均呈现良好的线性关系(r2≥0.9992);检测限与定量限分别为0.07-0.32和0.16-0.93μg·mL-1;日间与日内偏差小于0.45%;平均回收率在99.31%-100.19%,RSD范围为0.29%-1.28%。结论:本方法可用于玄参生品与炮制品内在质量控制。 |
| 关 键 词： | 高效液相色谱-二极管阵列检测器 玄参 炮制 质量控制 |
Simultaneous quantitation of eight active components in crudeand processed Radix Scrophulariae extracts by high performanceliquid chromatography with diode array detector |
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| CAO Gang,CONG Xiao-Dong,CAI Hao,＊,LI Xiao-Meng,JI Jin-Yu,ZHANG Yun,CAI Bao-Chang. Simultaneous quantitation of eight active components in crudeand processed Radix Scrophulariae extracts by high performanceliquid chromatography with diode array detector[J]. Chinese JOurnal of Natural Medicines, 2012, 0(3): 213-217 | |
| Authors: | CAO Gang CONG Xiao-Dong CAI Hao ＊ LI Xiao-Meng JI Jin-Yu ZHANG Yun CAI Bao-Chang |
| Affiliation: | 1,2,3,4＊1 Research Center of TCM Processing Technology,Zhejiang Chinese Medical University,Hangzhou 310053,China;2 College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China;3 Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of ChineseMedicine,Nanjing 210029,China;4 Nanjing Haichang Chinese Medicine Group Corporation,Nanjing 210061,China |
| Abstract: | AIM：To determine eight major components in crude and processed Radix Scrophulariae,several samples from differentarrears were examined.METHODS：The eight components were separated on an Agilent Zorbax Extend C18 column（250 mm × 4.6 mm,5 μm） and detected by diode array detector（DAD）.Mobile phase was composed of（A） aqueous phosphoric acid（0.03%,V/V） and（B）acetonitrile using a gradient elution.Analytes were performed at 30 °C with a flow rate of 1.0 mL·min-1 and UV detection at 280 nm and210 nm.RESULTS：All calibration curves showed good linear regression（r2 ≥ 0.999 2） within tested ranges.The limits of detectionand limits of quantification were 0.07-0.32 μg·mL-1 and 0.16-0.93 μg·mL-1,respectively.Overall intra-day and inter-day variationswere less than 0.45%,and the average recoveries were 99.31%-100.19%,with RSD ranging from 0.29% to 1.28% for the analytes.CONCLUSION：The developed method can be applied to the intrinsic quality control of crude and processed Radix Scrophulariae. |
| Keywords: | HPLC-DAD Radix Scrophulariae Processed Quality control |
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