染色法快速鉴别湖北钉螺死活的实验研究

目的 目的 寻找一种快速准确、 简便易行的鉴别湖北钉螺死活的方法。方法 方法 分别用0.05%中性红水溶性染液、 0.5%甲基蓝染液、 红墨水、 伊红亚甲基蓝 （MEB） 染液、 0.4%台盼蓝对活、 死钉螺进行染色， 染色30 min后压碎螺壳， 体视显微镜下观察软体组织着色情况。结果 结果 0.05%中性红可将活钉螺均染成红色， 死钉螺均未着色； 0.5%甲基蓝染色后， 活、 死钉螺均不着色； 红墨水可将活、 死钉螺均染成红色； MEB染液可使部分活、 死钉螺着色； 0.4%台盼蓝可将活钉螺和部分死钉螺均染成蓝色。结论 结论 使用0.05%中性红水溶性染色液对钉螺进行染色处理， 可快速、 准确、 客观地鉴别钉螺死活。

Experimental study on quick determination of viability of Oncomelania hupensis by staining

Objective To find out a quick，simple and convenient method of determining the viability of Oncomelania hupen?sis. Methods O. hupensis snails were stained for 30 minutes by 0.05% water soluble dye neutral red，0.5% methylene blue，redink，methylene blue?eosin?borax（MEB）and 0.4% trypan blue，respectively. The soft tissue samples of the snails were observedby a stereoscopic microscope after crushing their shells. Results The vital snails were stained and the dead were unstained in theneutral red. The vital and dead snails were unstained in methylene blue. However，the vital and dead snails were stained in redink. The partial vital and dead snails were stained in MEB. The vital snails were stained and the partial dead were stained in trypanblue. Conclusion The use of 0.05% water soluble dye neutral red is simple，rapid and accurate in determination of the viabilityof O. hupensis.