干燥保存的猴眼角膜穿透移植实验

Grafting experiment of preserved cornea of rhesus monkeys

The corneas preserved by desiccation for 10-57 days were used in penetrating keratoplasties in 9 eyes of the monkeys, with transparent healing in 3 eyes, semitransparent in 2 eyes while opaque in 4 eyes. The desiccated corneas of monkeys were examined after vital stain. The cellular profiles were not clear but the nuclei were distinct with their density of distribution similar to that of the fresh cornea. Ultrastructure showed pyknosis ofchromatin, essentially complete nucleus membrane and abundant organellae. The results suggested that, before transplantation, the endothelial cells of the corneas of monkeys preserved by desiccation were different from those of fresh cornea, but the basic structures essential to the existence of the cells still existed and, after transplantation, the cells could revive under the cultivation by the aqueous humor with their morphology and function returned to normal. The experiment provided the evidence of feasibility in using corneas preserved by desiccation in penetrating keratoplasty to save the eyeball.