赫赛汀对鼻咽癌荷瘤裸鼠肿瘤生长影响的实验研究

目的：探讨赫赛汀（Herceptin）对HER-2/neu基因蛋白高表达的鼻咽癌荷瘤裸鼠肿瘤生长的影响.方法：用鼻咽癌CNE-2Z细胞株接种于裸小鼠右腋下建立HER-2/neu基因蛋白高表达的裸鼠移植瘤模型.将已构建模型的裸鼠24只随机分为4组,每组6只,分别设阴性和阳性对照两组,Herceptin 10mg/kg和20mg/kg两实验组,以观察Herceptin对移植瘤体内生长的影响.结果：用Herceptin处理后的裸鼠移植瘤肿瘤体积均低于阴性对照组,其中10mg/kg剂量组与阴性对照组相比有统计学差异（P〈0.05）,但无量效关系;瘤重抑制率Herceptin 10mg/kg和20mg/kg分别为33.7%和23.3%,均未达到阳性标准;对裸小鼠生长无明显影响.结论：Herceptin对HER-2/neu基因过表达的鼻咽癌细胞株移植的荷瘤裸鼠肿瘤体内的生长具有一定的抑制作用,但效果并不满意.

The experimental study of Herceptin on the tumor growth of nasopharyngeal carcinoma xenografts tumor nude mice

Objective:To study the effect of Herceptin on tumor growth of xenografts with nasopharyngeal carcinoma(NPC) cells line overexpressed HER-2/neu gene.Methods:CNE-2Z NPC cells line were inoculated right armpit of nude mice to construct xenografts tumor model of NPC with HER-2/neu gene protein overexpression.Twenty-four nude mice constructed model were randomly divided into four groups,including negative and positive groups,10mg/kg and 20mg/kg Herceptin experimental groups,mice numbers were six in every group.The effect of Herceptin on tumor growth of xenografts in vivo was observed.Results:The xenografts tumor volum was less in Herceptin groups than in negative control groups,there was statistically significant difference among 10mg/kg Herceptin groups compared with negative control groups(P<0.05),but dose-effect relationship had not been observed;the inhibit rate of tumor weight were 33.7% and 23.3% in 10mg/kg and 20mg/kg Herceptin groups,but the results were not satisfied with positive standard.Herceptin had not been the effect of nude mice growth.Conclusion:Herceptin had the inhibitory effect on tumor growth of xenografts with NPC cells line overexpressed HER-2/neu gene in vivo,but the inhibitory effect was not satisfied.