牛磺酸对苯并(a)芘致L-02细胞损伤保护作用

目的 研究牛磺酸对苯并(a)芘(B(a)P)致人胚肝细胞(L-02细胞)染色体损伤的保护作用.方法 以L-02细胞为靶细胞,按完全随机设计分成5组:(1)空白对照组;(2)溶剂对照组;(3)B(a)P染毒组:设12.5,25,50μmol/L3个剂量染毒细胞;(4)牛磺酸组:设1.0,2.0,4.0mmol/L3个浓度,加入培养体系后继续培养24h;(5)牛磺酸预防组:L-02细胞先以3个不同浓度的牛磺酸预处理24h,再加入25μmol/L的B(a)P培养24h.各组均设3个平行样.收获细胞后用微核实验检测染色体损伤,计算微核率和核分裂指数.结果 牛磺酸单独作用于L-02细胞时,与空白对照组比较,中、高浓度牛磺酸诱导的微核率降低,核分裂指数增高,差异有统计学意义.从低到高3个剂量B(a)P单独染毒L-02细胞诱导的微核率为(34.67±2.52),(45.33±4.16),(60.00±7.21)%.核分裂指数分别为1.326±0.034,1.228±0.006,1.148±0.012,与溶剂对照组比较微核率升高,核分裂指数降低,差异有统计学意义,且两者均呈明显的剂量-反应关系.低、中、高浓度牛磺酸预防组诱导的微核率对应为(30.33±3.51),(25.67±1.53)和(23.00±1.00)%.均比单纯25μmol/LB(a)P染毒组诱导的微核率降低,差异有统计学意义.低、中、高浓度牛磺酸预防组对应的核分裂指数为1.455±0.011,1.474±0.015和1.1492±0.013,比单纯25μmol/LB(a)P染毒诱导的核分裂指数高,差异有统计学意义,且两者均呈剂量-反应关系.结论 B(a)P能诱导肝细胞染色体损伤,且存在明显的剂量-反应关系;牛磺酸预处理对B(a)P引起的肝细胞毒性具有明显保护作用.

Effect of taurine on micronuclei formation of L-02 cells exposed to benzo(a)pyrene

Objective To investigate the protective effect of taurine on chromo some damage of human derived fetal hepatocytes(L-02 cells)induced by benzo(a)pyrene(B(a)P).Methods L 02 cells were randomized divided into 5 groups:blank control group(Ⅰ);solvent control group(Ⅱ);B(a)P group(Ⅲ):exposed to B(a)P at concentration of 12.5,25,50 mol/L,in culture medium for 24 ho urs;taurine group(IV):exposed to taurine at the concentration of 1.0,2.0,4 0mmol/L in culture medium for 24 hours;taurine preventive group(V):exposed to taurine at three different concentration in culture medium for 24 hours and treated further for 24 hours by add ing 25 mol/L B(a)Pinto the culture media.Chromo some damage was detected by cytok inesis block mic ronucleus assay(CBMNT).The frequencies of MN(%)and nucleidivision index(NDI)were calculated.Results Compared with the blank control,taurine at concentrations of 2.0 and 4.0mmol/L induced a significant decrease in M N frequencies and an increase in NDI in L-02 cells.Compared with the solvent control,B(a)Pat concen trations of 12.5,25 and 50 mol/Linduced a significant enhancement of MN frequencies and reduce in ND I.Compared with the 25 mol/LB(a)P group,pretreatment with taurine could induce a significant decrease in M N frequencies and an increase in NDI in L-02 cells.The reductions of MN frequency and NDI showed a dose effect manner.Conclusion B(a)P could induced chromo some damage in dose effectmanner.Taurine could play a protective role in hepaticcy to toxicity induced by B(a)P.