5-氟胞嘧啶耐药白色念珠菌的DNA与RNA的随机扩增多态性分析

目的:比较对5-氟胞嘧啶耐药与敏感的白色念珠菌DNA基因组与RNA表达,探讨复发性念珠菌阴道炎的耐药机理.方法:选择临床诊断为复发性念珠菌阴道炎的病例16例,取阴道分泌物培养,提取菌株DNA基因组,应用随机扩增多态性DNA法分析菌株的DNA基因组的多态性,对分离菌株进一步做药敏试验,获得白色念珠菌耐药菌株与敏感菌株各8株;提取菌株RNA,利用随机扩增多态性RNA方法检测基因组的RNA表达.结果:临床耐药菌株与敏感菌株的DNA基因组指纹图无明显差异,但基因组的RNA表达存在差异,与敏感菌株比较,耐药菌株基因组的RNA出现条带数目的缺失或位置的改变.结论:复发性念珠菌阴道炎耐药的原因之一是表型耐药,而非基因型耐药,敏感菌株与耐药菌株共有一套基因组,在致病环境发生改变时,基因组RNA的表达转换,导致临床上表现出可逆耐药而非稳定耐药.

DNA and RNA random amplification polymorphism in 5-flurocytosine-resistant strains of Candida albicans from revul-vaginal candidasis

Objective: To investigate the DNA genome and RNA expression in 5 flurocytosine resistant strains of Candida albicans from vaginal candidasis. Methods: Sixteen strains of Candida albicans were selected from clinically diagnosed revul vaginal candidasis. Eight 5 flurocytosine sensitive isolates and 8 resistant isolates were exa mined by France Media FUNGUS sensitive test. DNA genome was detected with random amplification polymorph DNA. RNA expression was detected with random amplification polymorph RNA method. Results: There were no distinct differences between 5 flurocytosine sensitive and resistant Candida albicans in DNA genome, while RNA expression showed significant differences between 5 flurocytosine resistant and sensitive strains. Conclusion: Cli nical 5 flurocytosine resistant strains of Candida albicans from revul vaginal candidasis may be related to phenotype changes.