治疗视网膜脉络膜新生血管的竹红菌乙素光动力学疗法实验系统的建立

目的 建立一种治疗视网膜脉络膜新生血管的竹红菌乙素光动力学疗法实验系统。方法 1.在裂隙灯显微镜下开创激光窗口，光纤连接激光器和裂隙灯显微镜，使激光和裂隙灯照明同光路到达眼底，作为照射系统；2．通过四甲偶氮唑盐(MTT)法检测光敏剂竹红菌乙素(HB)体外光敏性，和苯并卟啉衍生物单酸A环(BPD-MA)相比，初步判断其光敏性；3．以光敏剂HB为例，青紫蓝兔为实验对象，进行PDT治疗，通过眼底观察，荧光眼底造影、光学显微镜和电子显微镜检查照光部位的生物学效应，检测建立的实验系统的实用性，可靠性。结果 照射系统输出稳定，由激光器发出的功率和裂隙灯显微镜的激光窗口末端的输出的功率的耦合率为60％。国产HB体外光敏性和BPD-MA相似。HB-PDT仅对脉络膜毛细血管造成损伤而对周围正常组织损伤小，达到选择性治疗目的，有进一步研究价值。结论 新建立的照射系统和光敏剂光敏性体外评价系统，以及体内观察生物学效应的方法，可作为初步判定光敏剂是否适合治疗视网膜或脉络膜新生血管的方法。

Establishment of Experimental System of Hypocrelline B Photodynamic Therapy for Treatment of the Retina and Choroidal Neovascularization(NV)

Objective To establish an experimental system of hypocrelline B photodynamic therapy (PDT) for treatment of the retina and choroid neovascularization. Methods 1.To create the irradiation system: a laser window was created under the split microscope to enable 532nm-laser and the irradiation light of the split microscope to arrive at the fundus at the same time 2.The sensitivity in vitro of the photosensitizer hypocrelline B was determined by MTT in comparing with benzoporphyrin derivative monoacid A ring (BPD-MA) 3.The system was testified by domestic photosensitizer hypocrellin B photodynamic therapy (HB-PDT) The biological effects at the irradiated area were evaluated by fundus observation, fluorescein angiography and histological examination for determination of applicability and reliability of the system Results The output of the laser was stable and the coupling rate between the laser and laser window at the split microscope was about 60% The sensitivity of HB in vitro was similar to BPD-MA, and HB-PDT could lead to closure choriocapillaris with minimal damage to the normal retina Conclusions It is a useful tool to decide whether a new photosensitizer is fit to treat neovascularization by using the irradiation system, the method of determination for in vitro photosensitivity of the photosensitizer and biological effect in vivo