| 宁波市江北区婴幼儿腹泻病原分析 |
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| 引用本文： | 徐奋奋,徐景野,裘立晓,陈美芬,金晓霞,蔡颖. 宁波市江北区婴幼儿腹泻病原分析[J]. 中国卫生检验杂志, 2010, 0(1) |
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| 作者姓名： | 徐奋奋 徐景野 裘立晓 陈美芬 金晓霞 蔡颖 |
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| 作者单位： | 浙江省宁波市江北区疾病预防控制中心;浙江省宁波市疾病预防控制中心; |
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| 摘 要： | 目的:了解宁波市江北区婴幼儿腹泻病原组成及其流行情况,为腹泻病的防治提供依据。方法:采集2007~2008年宁波市江北区腹泻患儿粪便265份,采用细菌培养法检测6种肠道致病菌:沙门菌、志贺菌、霍乱弧菌、副溶血性弧菌、大肠杆菌O157:H7、致病性气单胞菌。采用酶联免疫吸附试验(ELISA法)快速检测粪便样本中轮状病毒,通过聚丙烯酰胺凝胶电泳(PAGE)检测轮状病毒核酸。采用逆转录-聚合酶链反应(RT-PCR)方法扩增并检测轮状病毒。结果:在265份样品中,检出肠道致病菌2株,致病菌感染率为0.75%。用ELISA法和RT-PCR法检出轮状病毒阳性样本分别为86株和84株,阳性率分别为33.21%和31.70%,两者检测结果无统计学差异。PAGE法结果显示均为A组轮状病毒,未发现病毒细菌混合感染现象,而2岁以下腹泻病人,轮状病毒检出率为78.57%。结论:轮状病毒是江北区婴幼儿腹泻最主要的病原体,且以轮状病毒A组为主,2岁以下儿童是轮状病毒感染的高危人群,应加强对腹泻儿童轮状病毒的监测。
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| 关 键 词： | 婴幼儿 腹泻 轮状病毒 肠道致病菌 |
Pathogenic analysis of infants and children with diarrhea in Jiangbei District of Ningbo City |
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| XU Fen-fen,XU Jing-ye,QIU Li-xiao,CHEN Mei-fen,JIN Xiao-xia,CAI Ying. Pathogenic analysis of infants and children with diarrhea in Jiangbei District of Ningbo City[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(1) |
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| Authors: | XU Fen-fen XU Jing-ye QIU Li-xiao CHEN Mei-fen JIN Xiao-xia CAI Ying |
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| Affiliation: | XU Fen-fen1,XU Jing-ye2,QIU Li-xiao1,CHEN Mei-fen1,JIN Xiao-xia1,CAI Ying1 (1.Jiangbei Center for Disease Control , Prevention,Ningbo 315020,China,2.Ningbo Center for Disease Control , Prevention,Ningbo 315010,China) |
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| Abstract: | Objective:To learn the pathogenic components and epidemic situation of infants and children with diarrhea in Jiangbei District,Ningbo City;to provide grounds for preventive measures of diarrhea.Methods:Collect 265 samples of feces of infants and children with diarrhea in Jiangbei District through 2007 to 2008,Culturing six major intestinal pathogenic bacteria such as Salmonella,Shigella,Comma bacillus,E.coli O157:H7.Then apply the method of ELISA for instant detection of rotavirus;apply the method of PAGE t... |
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| Keywords: | Infants and children Diarrhea Rotavirus Intestinal pathogenic bacterial |
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