| 溶栓素的纯化研究 |
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| 引用本文： | 程熠,史方亮,王之光,洪敏.溶栓素的纯化研究[J].吉林大学学报(医学版),2001,27(1):9-11. |
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| 作者姓名： | 程熠 史方亮 王之光 洪敏 |
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| 作者单位： | 1. 吉林大学基础医学院生物化学与分子生物学教研室,
2. 吉林康威药业 |
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| 基金项目： | 吉林省计委科研项目;9905G168; |
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| 摘 要： | 目的 :研究溶栓素的纯化工艺。方法 :采用超滤法截留分离 ,强阴离子交换层析去除核酸 ,弱阴/阳离子交换层析和疏水层析组合法精细分离溶栓素。结果 :聚丙烯酰胺凝胶电泳考马斯亮蓝染色法结合显示最终纯化产物为一条染色带 ,纯化倍数为 144 .83,回收率为 38.6 6 %。结论 :溶栓素的纯化工艺基本体现了工业生产工艺的特点 ,具有一定的实用性。
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| 关 键 词： | 溶栓素 纤溶酶 纯化 |
| 文章编号： | 0253-3707(2000)01-0009-03 |
| 修稿时间： | 2000年1月20日 |
Isolation and purification of thrombolysin |
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| CHENG Yi,SHI Fang-liang,WANG Zhi-guang,HONG Min.Isolation and purification of thrombolysin[J].Journal of Jilin University: Med Ed,2001,27(1):9-11. |
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| Authors: | CHENG Yi SHI Fang-liang WANG Zhi-guang HONG Min |
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| Institution: | CHENG Yi~1,SHI Fang-liang~1,WANG Zhi-guang~2,HONG Min~1 |
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| Abstract: | Objective:The purification process of thrombolysin was researched.Methods:Ultrafiltration, ionexchange chromatography,and hydrophobic-interation chromatography were used.Results:A single band of final purification product was displayed in PAGE (Coomassic Brilliant Blue Stain Method).Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.The purification process had practicability. |
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| Keywords: | thrombolysin purification chromatography |
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