卵巢浆液性肿瘤中OCT4蛋白的表达及与化疗耐药的关系研究

目的:探讨OCT4蛋白在卵巢浆液性肿瘤组织中的表达与卵巢肿瘤发生发展及与化疗耐药的关系。方法:采用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法（SP法）检测OCT4蛋白在10例正常卵巢组织、10例卵巢良性浆液性囊腺瘤、10例卵巢交界性浆液性囊腺瘤和67例卵巢浆液性腺癌（其中化疗耐药30例，化疗敏感37例）的石蜡病理切片中的表达情况。结果:OCT4蛋白的阳性表达率在正常卵巢组织、卵巢良性浆液性囊腺瘤、卵巢交界性浆液性囊腺瘤和卵巢浆液性腺癌组织中分别为0、40.00%、50.00%和62.69%，差异有显著统计学意义（P=0.002）。在卵巢浆液性腺癌化疗耐药和化疗敏感组织中分别为83.33%和45.95%，差异有显著统计学意义（P=0.002）。OCT4蛋白的阳性表达率与卵巢浆液性腺癌的临床病理特征无明显相关性（P＞0.05）。结论:OCT4蛋白可能在卵巢浆液性肿瘤发生发展和卵巢癌化疗耐药中发挥重要作用，可能作为卵巢癌化疗耐药预测的一个重要参考指标。

Expression of OCT4 and correlation with chemotherapy resistance in ovarian serous neoplasms

Objective:To investigate the relationship of OCT4 protein expression with occurrence of ovarian serous neoplasms and chemoresistance of ovarian cancer.Methods:OCT4 protein expression in 10 cases of normal ovarian tissues,10 cases ovarian serous cystadenoma,10 cases ovarian borderline serous cystadenoma and 67 cases ovarian serous cystadenocarcinoma（30 cases chemotherapy resistance;37 cases chemotherapy sensitive) were detected by immunohistochemical SP method.Results:The positive rates of OCT4 in normal ovarian tissues,ovarian serous cystadenoma,ovarian borderline serous cystadenoma and ovarian serous cystadenocarcinoma were 0,40.00%,50.00% and 62.69%（P=0.002),in chemotherapy resistance and chemotherapy sensitive tissues were 83.33% and 45.95%（P=0.002).The positive rates of OCT4 did not associate with clinical-pathological data of ovarian cancer（P＜0.05).Conclusion:OCT4 correlates to occurrence and chemoresistance of ovarian serous cancer,and may be considered as one of indexes for predicting prognosis and chemoresistance.