动员造血干细胞对局灶性缺血再灌注大鼠脑组织C-FOS mRNA表达的影响

目的探讨应用粒细胞集落刺激因子(G-CSF)动员自体造血干细胞(HSC)对大脑中动脉栓塞/再灌注大鼠(MCAO/R)脑组织C-FOSmRNA表达的影响。方法制作MCAO/R模型;免疫组化双标记法鉴定HSC向神经元样细胞分化;RT-PCR法检测C-FOSmRNA表达水平。结果模型 G-CSF组再灌注后24 h出现CD34/Brdu双标记,48 h时双标记最为显著;再灌注后48 h出现CD34/NSE双标记。模型组各时间点C-FOSmRNA表达显著高于假手术组;G-CSF致再灌注48 h后C-FOSmRNA表达显著下调。结论应用G-GSF动员大鼠自体HSC可促进脑缺血再灌注损伤修复,调节C-FOSmRNA表达水平可能为其作用机制之一,而部分HSC来源的细胞分化为神经元样细胞可能参与缺血损伤修复过程。

Effects of mobilized hematopoietic stem cells on the expression of C-FOS mRNA in focal cerebral ischemia/reperfusion rats

Objective To study the effect of autologous hematopoietic stem cells mobilized by G-CSF on the expression of C-FOS mRNA in focal cerebral ischemia/reperfusion(MCAO/R) rats.Methods MCAO/R was induced by the filament occlusion method.Immunohistochemisty and dual labeling technique were used to detect HSCs differentiating into neuron-like cells after focal cerebral ischemia.RT-PCR technique was used to detect the expression of C-FOS mRNA.Results In the G-CSF treatment group,24 hours after the focal cerebral ischemia/reperfusion injury,CD34/Brdu dual labeled cells were found in the ischemia area;48 hours later,CD34/Brdu and CD34/NSE dual labeled cells were found,Compared with the control group,there was an enhanced expression level of C-FOS mRNA in the model group; In contrast,the expression of C-FOS mRNA in the G-CSF treatment group reduced.(Conclusion Autologous) hematopoietic stem cells mobilized by G-CSF can restore injury and decrease the expression of C-FOS mRNA.Some of HSCs can differentiate into neuron-like cells and may play an important role in the progress of the restoration.