2006年婴幼儿腹泻轮状病毒监测分析

目的监测乌鲁木齐地区婴幼儿腹泻轮状病毒（RV）感染情况和流行特点。方法 2006年1月至12月,采集门诊或住院≤5岁婴幼儿腹泻样本248例,用酶免法（ELISA）筛查RV,用RT-PCR方法对RV阳性毒株进行G基因型分型。采用SPSS13.0统计软件,数据处理用χ2检验。P〈0.05表示统计学差异有统计学意义。结果 248例腹泻样本,检出RV 133例,阳性率为53.6%。RV感染对象主要为24月以内婴幼儿,占94.7%,发病高峰在8~9月份。对125份RV阳性毒株进行G基因型分型,结果显示：G1型（46.4%）为最流行毒株,其次为G3型（17.6%）、G9型（7.2%）和G2型（4.0%）,混合感染占14.4%,不能分型占10.4%。结论轮状病毒是乌鲁木齐地区婴幼儿腹泻的主要病原,2006年G1和G3型为主要流行基因型。

Rotavirus surveillance in children with diarrhea

Objective To investigate the characteristics of rotavirus infection among infants and children with diarrhea in Urumqi.Methods 248 fecal samples were collected from children under 5 years old with diarrhea and examined for rotavirus antigen by ELISA in 2006.Rotavirus strains were genotyped by RT-PCR with primers selected from G gene area.Results Rotavirus was detected in 133（53.6%） diarrheal children.94.7% of patients with rotavirus diarrhea occurred among children less than 24 months old.The peak season was August through September.The predominant strain was G1（46.4%）,followed by G3（17.6%）,G9（7.2%） and G2（4%）.14.4% of patients had mixed infection and 10.4% remained undetermined for strain typing.Conclusion Rotavirus was the major cause of diarrhea in young children in Urumqi.G1 and G3 were the most prevalent genotypes in 2006.