两种胶体金免疫层析诊断试剂检测轮状病毒效果比较

目的比较两种A组轮状病毒胶体金免疫层析诊断试剂的灵敏度和特异度。方法用两种胶体金免疫层析试剂(万泰和拜发试剂盒)对166份腹泻患者粪便标本进行轮状病毒检测,与酶联免疫吸附试验(ELISA)的检测结果进行比较。结果以ELISA方法为参照,万泰公司胶体金试剂的敏感度为84.6%(33/39),特异度为96.1%(122/127),阳性预测值和阴性预测值分别为86.8%(33/38)和95.3%(122/128);拜发公司胶体金试剂的敏感度为82.1%(32/39),特异度为96.9%(123/127),阳性预测值和阴性预测值分别为88.9%(32/36)和94.6%(123/130)。结论本实验结果表明,胶体金免疫层析诊断试剂简便可靠,可用于A组轮状病毒的快速检测。

Evaluation of two colloidal gold immunochromatographic assays for detection of group A rotavirus

Objective To evaluate the diagnostic value of colloidal gold immunochromatographic assay for detection of group A rotavirus.Methods Two colloidal gold immunochromatographic assays（Wantai and R-Biopharm kits） were used to detect rotaviruses in stool specimens collected from 166 patients with acute diarrhea,and results of which were compared with the reports of an enzyme-linked immunosorbent assay（ELISA）.Results As compared with ELISA,the sensitivity,specificity,positive and negative predictive values were 84.6%（33/39）,96.1%（122/127）,86.8%（33/38） and 95.3%（122/128） for Wantai kit;and 82.1%（32/39）,96.9%（123/127）,88.9%（32/36） and 94.6%（123/130） for R-Biopharm kit,respectively.Conclusion The colloidal gold immunochromatographic assays were reliable and convenient,and may be used for the rapid detection of group A rotavirus.