心肌梗死后移植骨髓间充质干细胞后全身细胞的分布 |
| |
引用本文: | 王业焕,金培峰,袁昕,侯建峰,孙成超,胡盛寿,张浩. 心肌梗死后移植骨髓间充质干细胞后全身细胞的分布[J]. 中华实验外科杂志, 2008, 26(1): 914-916,插图7-3. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.037 |
| |
作者姓名: | 王业焕 金培峰 袁昕 侯建峰 孙成超 胡盛寿 张浩 |
| |
作者单位: | 中国医学科学院阜外心血管病医院卫生部心血管疾病再生医学重点实验室温州医学院第一附属医院心胸外科,北京,100037;温州医学院第一附属医院心胸外科;中国医学科学院阜外心血管病医院卫生部心血管疾病再生医学重点实验室,北京,100037; |
| |
基金项目: | 国家自然科学基金资助项目北京新星计划资助项目 |
| |
摘 要: | Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.
|
关 键 词: | 心肌梗死 细胞移植 细胞分布 |
Cell body distribution after bone marrow mesenchymal stem cells intramyocardial injection into a rat myocardial infarction model |
| |
Abstract: |
|
| |
Keywords: | Myocardial infarctionCell transplantationCell distribution |
|
|