| 酶联免疫法定量检测幽门螺杆菌抗体的研究 |
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| 引用本文： | 周燕 邓长生. 酶联免疫法定量检测幽门螺杆菌抗体的研究[J]. 临床消化病杂志, 1998, 10(1): 6-7 |
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| 作者姓名： | 周燕 邓长生 |
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| 作者单位： | 湖北医科大学第二医院消化内科 武汉430000(周燕，邓长生，易季云，黄梅芳)，湖北医科大学第二医院消化内科 武汉430000(肖建群) |
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| 摘 要： | 对73例十二指肠溃疡和胃炎进行血清抗HP抗体定量测定,结果以尿素酶试验和胃粘膜涂片同时阳性作为HP阳性标准,判断血清抗体法检测HP阳性符合率,以同样方法判断血清抗体法阴性符合率.在尿素酶和胃粘膜涂片阳性39例中,抗HP-IgG抗体阳性35例,阳性符合率为89%;尿素酶和胃粘膜涂片阴性34例中,30例阴性,阴性符合率为88%,5例在临界值范围.经卡方检验差别无显著性(P>0.05).抗HP-IgG定量方法的测定比定性方法更准确.定量方法可得出具体数据,可以判断患者感染细菌时间长短以及反映机体对HP免疫力强弱.HP血清学检查可用于观察抗HP疗效及HP普查.本操作每次应设阴阳性对照,用标准品作为定量标准,标本定量应在24h内完成,以免HP-IgG效价降低,若放置低温冰箱,应避免反复冻融.
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| 关 键 词： | 幽门螺杆菌 抗HP-IgG抗体 定量测定 尿素酶试验 胃粘膜涂片 |
Study on the Level of Anti-Helicobacter Pylori IgG Antibody with Enzyme Linked Immunosorbent Assay |
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| Zhou Yan,Deng Chang-sheng,Yi Ji-yun,et al. Study on the Level of Anti-Helicobacter Pylori IgG Antibody with Enzyme Linked Immunosorbent Assay[J]. Chinese Journal of Clinical Gastroenterology, 1998, 10(1): 6-7 |
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| Authors: | Zhou Yan Deng Chang-sheng Yi Ji-yun et al |
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| Affiliation: | Zhou Yan,Deng Chang-sheng,Yi Ji-yun,et alDepartment of Gastroenterology,Second Affiliated Hospital Hubei Medical University,Wuhan |
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| Abstract: | 73 patients of duodenal ulcer and chronic gastritis were evaluated for anti-Helicobacter pylori IgG antibody by enzyme-linked immunosorbent assay (ELISA). It was the positive standard of HP infection that urease test and gastric mucosal biopsy were positive together. The results showed that the sensitivity of anti-HP IgG antibody with ELISA method was 89% (35/39), the specificity was 88% (30/34), chi square test (x2 test) was not significantly difference (P >0.05). The conclusion suggested the method used to test anti-HP IgG antibody with ELISA was better than the qulitative methods. |
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| Keywords: | Helicobater pylori Anti-HP IgG antibody Enzyme-linked immunosorbent assay Urease test Gastric mucosal biopsy |
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