| 恶性疟原虫海南株CTP基因的克隆测序及序列分析 |
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| 引用本文： | 陈慧红 余新炳 等. 恶性疟原虫海南株CTP基因的克隆测序及序列分析[J]. 中国人兽共患病杂志, 2001, 17(6): 40-42 |
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| 作者姓名： | 陈慧红 余新炳 等 |
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| 作者单位： | 陈慧红（中山医科大学寄生虫学教研室,广州,510089） 余新炳（中山医科大学寄生虫学教研室,广州,510089） 吴忠道（中山医科大学寄生虫学教研室,广州,510089） 陆家海（中山医科大学寄生虫学教研室,广州,510089） 徐劲（中山医科大学寄生虫学教研室,广州,510089） |
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| 基金项目： | 本课题获得广东省"211工程"重点建设学科基金和国家教委博士点基金的资助 |
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| 摘 要： | 目的 测定恶性疟原虫（P.f）海南株CTP基因序列，了解该虫株CTP基因序列与其它种类的CTP基因序列的差异，用于药物靶位的筛选。方法 用PCR的方法特异性的扩增CTP基因，并将此基因克隆于测序载体pUC19，采用Sanger双脱氧链末端终止法测序。结果 我国海南株的CTP基因序列与其它的CTP基因有不同程度的差异。结论 P.f CTP作为潜在的抗疟药物靶值得进一步深入研究。
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| 关 键 词： | 恶性疟原虫 CTP基因 基因测序 |
| 文章编号： | 1002-2694(2001)06-0040-03 |
| 修稿时间： | 2001-08-08 |
CLONING AND SEQUENCING OF CTP GENE OF PLASMODIUM FALCIAPRUM HAINAN ISOLATEFCC1/HN |
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| CHEN Huihong,YU Xinbing,WU Zhongdao,LU Jiahai,XU Jin. CLONING AND SEQUENCING OF CTP GENE OF PLASMODIUM FALCIAPRUM HAINAN ISOLATEFCC1/HN[J]. Chinese Journal of Zoonoses, 2001, 17(6): 40-42 |
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| Authors: | CHEN Huihong YU Xinbing WU Zhongdao LU Jiahai XU Jin |
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| Abstract: | Aim To determine the sequence of CTP gene of Plasmodium falciparum and find out the differences of the CTP gene sequence between isolate Hainan and other isolates.Methods CTP gene was amplified by polymerase chain reaction and cloned into sequencing vector pUC19.The recombinant plasmid pUC19/CTP was screened by blue-white plaque and identified by PCR and restriction analysis.The cloned CTP genes were sequenced by Sanger's method.Results There is no significant differences between P.f CTP genes of FCC1/HN and pf K1 strain,but there is obvious difference between P.f CTP genes of FCC1/HN and human CTP.Conclusion It is valuable to further study on P.f CTP as a candidate target of anti-malaria drug. |
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| Keywords: | Plasmodium falciparum CTP gene Sequencing Human |
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