白细胞粘附作用对ELISA检测HBsAg干扰现象的探讨

目的 探讨在ELISA检测HBsAg试验中，标本混入WBC后，WBC的粘附现象对试验结果的干扰。方法 观察WBC粘附现象对测定结果的干扰情况，以及血浆中WBC含量与干扰程度的关系，同时采用增加洗涤次数的方法对抗WBC粘附的效果观察。结果 标本混入WBC，WBC在反应板内发生粘附。WBC的中性粒细胞含过氧化物酶对试验结果造成干扰；标本中单位体积WBC含量与WBC的粘附量不成正比，但WBC的有效粘附数与OD值直接相关，粘附量越多，OD值越高；且WBC的粘附经洗涤10次也未能完全洗脱。结论 在ELISA检测HBsAg工作中应注意WBC对试验产生的非特异性干扰，采用多种方法防止WBC的混入。

Interference in HBsAg Test(ELISA)from Adherence of Leucocytes

Objective To elucidate the interference in HBsAg test results by using ELISA from the adherence of leucocytes. Methods The study was designed to observe the influence of the adherence of leucocytes mixed into plasma samples on the HBsAg test results, the relationship between leucocyte counts and the intensity of interference, and effects of different washing times on the ELISA test results. Results As leucocytes were mixed into the plasma samples, the adherence occurred on the reaction plates. Perioxidase contained in neutralgranulocytes was suggested responsible for being the cause of adherence. However, leucocyte counts in unit volume of samples showed improportion to the intensity of adherence, but only correlated with the OD values. In addition, leucocyte adherence could not be fully eluded by increasing washing up to 10 times. Conclusion It should be cautious of the nonspecific interference in the HBsAg test from the formation of leucocyte adherence, and certain measures should be taken to avoid blending leucocytes into the test samples.