维生素K环氧化物还原酶复合体1基因多态性对华法林维持剂量的影响

目的 检测维生素K环氧化物还原酶复合体1（VKORC1）基因多态性对华法林维持剂量的影响。方法 研究入选102例使用华法林的汉族人,用限制性酶切的方法检测其C1173T位点多态性,其他临床资料通过随访获得。用独立样本t检验比较不同基因型患者华法林维持剂量的差别,用多重线性回归进行回顾性研究,分析华法林抗凝疗效的影响因素。结果 102例患者的C1173T位点基因型分别为TT基因型83个（81．4％）、CT基因型16个（15．7％）、CC基因型3个（2．9％）。在相同的抗凝目标下国际标准化比值1．5—2．5,TT基因型患者所需华法林剂量明显低于CT／CC基因型,分别为（2．54±0．83）mg／d、（4．19±1．16）mg／d（P〈0．01）。结论 C1173T位点多态性对华法林维持剂量有明显的影响,它能解释大约40％的个体间剂量差异（r^2=0．401）。

Vitamin K epoxide reductase complex 1 gene polymorphism and warfarin dose requirement in Chinese patients

OBJECTIVE: To investigate the possible relationship between vitamin K epoxide reductase complex 1 (VKORC1) gene polymorphism and warfarin dose requirements in Chinese patients. METHOD: Genotyping for the C1173T polymorphism in the VKORC1 gene was performed using a restriction enzyme digestion of PCR-amplified DNA in 102 Chinese patients treated with warfarin. RESULTS: TT genotype was found in 83 patients (81.4%), CT genotype in 16 patients (15.7%) and CC genotype in 3 patients (2.9%). To achieve similar target INR range (1.5 - 2.5), the warfarin dose requirement was significantly lower in patients with TT genotype than that in patients with CT/CC genotypes (P < 0.01). CONCLUSION: C1173T polymorphism in the VKORC1 gene might be one important determinant for warfarin dose requirement in Chinese patients.