赛庚啶对家兔脊髓缺血性损伤的保护作用

目的 :利用家兔脊髓缺血性损伤模型探讨赛庚啶对脊髓缺血性损伤的保护作用。方法 :2 4只家兔随机分为假手术组 (A组 )、缺血组 (B组 )和赛庚啶组 (C组 ) ,每组 8只。肾下阻断腹主动脉 4 0min后松开 ,C组于阻断前10min静注赛庚啶 2mg/kg ,继以 2mg/kg·h持续输注至松开前 10min。测定给药前、松开前及松开后 6 0min兔血浆中丙二醛 (MDA)含量。术后观察动物后肢神经功能、肌电图 (EMG)和脊髓形态学变化。结果 :B组主动脉阻断及再通后血中MDA含量明显升高 ,p <0 0 1,C组动物阻断及再通后无明显变化并明显低于B组值 ,C组动物术后瘫痪数低于B组 ,术后神经功能评分及EMG检查显示C组脊髓功能损伤明显轻于B组 ;B组脊髓病理变化明显 ,可见大量神经元坏死 ,C组偶见神经元变性 ,病理学评分两组有明显差异。结论 :赛庚啶对家兔主动脉阻断导致的脊髓缺血性损害有一定的保护作用 ,这一作用与赛庚啶抗过氧化反应及其他多种药理作用有关

The protective effects of cyproheptadine on ischemia injury of spinal cord in rabbits

Objective: To evaluate the protective effects of cyproheptadine on ischemia injury of spinal cord in rabbits. Methods: Twenty four rabbits were randomly divided into three groups: sham operation group(A), control group(B) and cyproheptadine group (C). The infrarenal aorta was crossclamped for 40 minutes and then teperfused. In group C, cyproheptadine (2 mg/kg) was given 10 minutes before cross clamping, followed by 2 mg/kg an hour infusion for 40 minutes. MDA content in plasma was assayed before, during and after crossclamping. The neurological outcomes and the eletromyograms (EMG) of hindlimb were evaluated after operation. The spinal cord was examined at the level of T 10 and L 3 for morphological changes 7days after operation. Results: In group B, both aortic clamping and declamping gave rise to a significant increase in the contents of MDA in plasma. In group C, the contents of MDA in plasma were significantly lower than those in group B. The neurological deficits in group B were significantly severe compared with that in group C. In group B, the pathological changes were obvious and great number neuronal death could be seen, while neuronal death was occasionally observed in group C. Conclusion: Cyproheptadine can protect spinal cord from ischemia reperfusion injury in rabbits. We interpret the protective mechanism as its antioxidation and other pharmacological effects.