A medically relevant capsular polysaccharide in Acinetobacter baumannii is a potential vaccine candidate |
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| Affiliation: | 1. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia;2. M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia;3. School of Molecular Bioscience, The University of Sydney, Sydney, Australia;4. School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia;5. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia;6. State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia |
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| Abstract: | Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection. |
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| Keywords: | Capsular polysaccharide Passive immunity Clinic population distribution |
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