Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains |
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| Affiliation: | 1. Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA;2. Roslin Institute, University of Edinburgh, Midlothian, Scotland, UK;1. Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone KT15 3NB, UK;2. Department of Microbial & Cellular Sciences, University of Surrey, Guildford GU2 7XH, UK;3. Institute of Virology and Immunology (IVI), Sensemattstrasse 293, CH-3147 Mittelhäusern, Switzerland;4. Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland;5. Department of Pathobiology, Institute of Immunology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna A-1210, Austria;1. Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA;2. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA;3. Department of Medical Microbiology, Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands;1. Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA;2. Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA;1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China;1. Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand;2. Interdisciplinary Program in Medical Microbiology, Graduate School, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand;3. Faculty of Veterinary Science, Chulalongkorn University, Pathumwan Bangkok 10330, Thailand;4. Center of Excellence in Emerging Infectious Diseases in Animals, Chulalongkorn University (CU-EIDAs), Pathumwan, Bangkok 10330, Thailand |
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| Abstract: | ![]()
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of arguably the most economically important global swine disease. The extensive genetic variation of PRRSV strains is a major obstacle for heterologous protection of current vaccines. Previously, we constructed a panel of chimeric viruses containing only the ectodomain sequences of DNA-shuffled structural genes of different PRRSV strains in the backbone of a commercial vaccine, and found that one chimeric virus had an improved cross-protection efficacy. In this present study, to further enhance the cross-protective efficacy against heterologous strains, we constructed a novel chimeric virus VR2385-S3456 containing the full-length sequences of shuffled structural genes (ORFs 3-6) from 6 heterologous PRRSV strains in the backbone of PRRSV strain VR2385. We showed that the chimeric virus VR2385-S3456 induced a high level of neutralizing antibodies in pigs against two heterologous strains. A subsequent vaccination and challenge study in 48 pigs revealed that the chimeric virus VR2385-S3456 conferred an enhanced cross-protection when challenged with heterologous virus strain NADC20 or a contemporary heterologous strain RFLP 1-7-4. The results suggest that the chimera VR2385-S3456 may be a good PRRSV vaccine candidate for further development to confer heterologous protection. |
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| Keywords: | Porcine reproductive and respiratory syndrome virus (PRRSV) Vaccine DNA shuffling Cross-protection Heterologous strains RFLP 1-7-4 |
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