Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo |
| |
| Institution: | 1. Center for International Health Research, Rhode Island Hospital, Brown University Medical School, Providence, RI 02903, USA;2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Minhang, Shanghai 200241, China;3. Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China;4. Department of Immunology, Research Institute of Tropical Medicine, Manila, Philippines;1. Adult Vaccines, Merck USA, West Point, PA, United States;2. Department of Health Policy, Vanderbilt University School of Medicine, Nashville, TN, United States;3. Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN, United States;1. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation (OMRF), 825 NE 13th St., Oklahoma City, OK 73104, USA;2. Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center (OUHSC), 940 Stanton L. Young Blvd, Oklahoma City, OK 73104, USA;3. Public Health England, National Infection Service, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom;4. Walter Reed National Military Medical Center, 8901 Wisconsin Ave, Bethesda, MD 20814, USA;5. Department of Pharmaceutical Chemistry, University of Kansas, 320B Multidisciplinary Research Building, 2030 Becker Dr., Lawrence, KS 66047, USA;6. Departments of Medicine and Pathology, OUHSC, 1000 Stanton L. Young Blvd, Oklahoma City, OK 73104, USA;1. Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA, USA;2. Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA;3. Department of Epidemiology and Control of Infectious Diseases, Institute of Public Health, Tirana, Albania;4. Abt Associates, Atlanta, GA, USA;5. The Eastern Mediterranean Public Health Network (EMPHNET), Amman, Jordan;6. Center for Global Health, Department of Epidemiology, Colorado School of Public Health, Aurora, CO, USA;7. Sustainable Sciences Institute, Managua, Nicaragua;8. Research Institute for Tropical Medicine, Department of Health, Muntinlupa, Philippines;9. Marshfield Clinic Research Foundation, Marshfield, WI, USA;10. Hospital Infantil Manuel de Jesus Rivera, Ministry of Health, Managua, Nicaragua;11. Laboratorio Nacional de Virologia, Centro Nacional de Diagnostico y Referencia, Ministry of Health, Managua, Nicaragua;12. Al Bashir Hospital, Ministry of Health, Amman, Jordan;13. General Pediatrics Unit, University Hospital Center “Mother Teresa”, Tirana, Albania;14. Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA;1. South Texas Center for Emerging Infectious Disease and the Center for Excellence in Infection Genomics, University of Texas at San Antonio; San Antonio, TX 78249, United States;2. Coagulation and Blood Research Program, US Army Institute for Surgical Research, JBSA Fort Sam Houston, TX 78234, United States;3. Comparative Pathology Division, US Army Institute for Surgical Research, JBSA Fort Sam Houston, TX 78234, United States;1. Griffith Health Institute, Griffith University, Gold Coast Campus, Southport, QLD, Australia;2. Research Institute for Tropical Medicine, Department of Health, Manila, Philippines;3. Hunan Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Control of Schistosomiasis in the Lake Region, Yueyang, Hunan, China;4. QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, People''s Republic of China;2. Key Laboratory on Biology of Parasite and Vector, Ministry of Health, People''s Republic of China, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People''s Republic of China;3. Department of Health, Research Institute for Tropical Medicine (RITM), Manila, Philippines;4. Tropical Disease Research Laboratory (TDR), Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;5. Jiangsu Institute of Parasitic Diseases, Wuxi 214064, People''s Republic of China;6. Key Laboratory on Control Technology for Parasitic Diseases, Ministry of Health, Wuxi 214064, People''s Republic of China;7. School of Public Health and Primary Care, The Jockey Club Chinese University of Hong Kong, Shatin, Hong Kong;8. College of Public Health, University of the Philippines, Manila, Philippines;9. Ingerod, Brastad, Sweden |
| |
| Abstract: | BackgroundSchistosomiasis japonica is a zoonosis and presents significant public health problems in China and the Philippines. Vaccines targeting domestic animals constitute attractive control measures.MethodsWe conducted three vaccine trials to evaluate the protective efficacy of recombinant full-length paramyosin (rSj97) in water buffalo. Animals were immunized with 3 doses of rSj97 adjuvanted with ISA206 at 250 μg/dose or 500 μg/dose at 4 wk intervals before challenge with 1000 Schistosoma japonicum cercariae. The primary outcome was worm burden assessed by portal perfusion 8–10 weeks post challenge. Safety measures included weight, temperature, body condition score, hemogram and routine assays for hepatic and renal function.ResultsThe three-dose regimen was well tolerated in all three trials. In the first trial, vaccinated buffalo had 51.5% lower worm burden post challenge compared to controls. In the second trial, buffalo immunized with 500 μg/dose of rSj97 had 57.8% lower worm burden compared to controls (p = 0.026). A similar but not significant reduction (60.9%) was observed with animals administered with 250ug rSj97/dose. In the third trial, buffalo immunized with a 500 μg/dose of rSj97 had 57.8% lower worm burden compared to controls (p = 0.014).ConclusionsThese findings indicated that rSj97 is a safe and promising vaccine candidate for schistosomiasis japonica in water buffalo. |
| |
| Keywords: | Paramyosin Vaccine Water buffalo |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
