反相高效液相色谱法测定人血浆中佐匹克隆的浓度

目的：建立高效液相色谱法测定血浆中佐匹克隆浓度。方法：采用Eurospher 100—5 C18色谱柱（200mm×4．6mm，5μm）；流动相：0．02mol·L^-1磷酸二氢钾（含0．2％三乙胺，pH=4．0）-甲醇（38：62）；流速：1．0ml·min^-1；柱温：3092；激发波长：315nm，发射波长：385nm。结果：血浆佐匹克隆在2．0～200．0ng·ml^-1浓度范围内线性关系良好（r=0．9995）。低、中、高3种浓度绝对回收率分别为92．64％，92．97％，94．66％，日内、日间RSD均小于10％。结论：本法可用于佐匹克隆血浆中浓度的测定以及药物动力学研究。

Determination the Concentration of Zopiclone in Human Plasma by HPLC

Objective： To establish an HPLC method for the determination of zopiclone in plasma. Method： The Eurospher 100-5 C18 column（200mm × 4.6mm,5 μm）was used. The mobile phase consisted of 0.02mol·L^-1 potassium dihydrogen phosphate-methanol （38： 62）. The flow rate was 1.0 ml·min^-1. The column temperature was 30℃. The excitation and emission wavelengths were 315nm and 385 nm, respectively. Result： The linear range of zopiclone was 2.0 -200.0ng·ml^-1（ r = 0.999 5）. The recoveries were 92.64%, 92.97% ,94.66% in the low ,meddle and high concentrations,respectively and RSD were below 10%. Condusion： The method can be used for the determination of zopiclone in plasma.