肺癌p53蛋白表达的免疫组织化学研究

应用链菌素亲生物素－过氧化物酶免疫组化方法研究了ｐ５３蛋白在６８例各型肺癌组织的表达情况。发现ｐ５３蛋白在常规石蜡切片中保存较好，在肺癌组织中表达的总阳性率为６３．２％，正常细胞中未见ｐ５３蛋白表达。提示ｐ５３蛋白是肺癌细胞的重要标志物。支气管粘膜上皮重度不典型增生的细胞可见ｐ５３蛋白表达，说明ｐ５３蛋白表达可能是支气管粘膜上皮癌变的早期事件。

Immunohistochemical Study of p53 Expression in Human Lung Carcinomas

mmunohistochemical analysis of p53 protein was carried out on 68 lung carcinomas from all histo-logical types in paraffin-embedded sections,using anti-p53 monoclonal antibody DO-1;Nuclear stainingwas seen in 63.2%of lung carcinomas of all histological types.No p53 expression was observed in nor-mal epithelial,stromal or inflammatory cells,but bronchial severe dysplasia showed p53 expression.These findings indicate that p53 protein may be a good marker of lung carcinomas and p53 protein ex-pression is an early event in the development of squamous cell carcinoma of lung.