参麦注射液抗肝缺血再灌注损伤的研究

目的：研究参麦注射液对肝缺血再灌注损伤的保护作用。方法：将３７只成年雄性ＳＤ大鼠随机分成假手术对照组（ＳＯＣ）、缺血再灌注组（Ｉ／Ｒ）、缺血再灌注加参麦组（Ｉ／Ｒ＋ｓｈｅｎｍａｉ）。通过阻断大鼠肝门３０ｍｉｎ后再开放建立肝缺血再灌注损伤模型，在肝脏再灌注９０ｍｉｎ时测肝组织丙二醛（ＭＤＡ）、超氧化物歧化酶（ＳＯＤ）和测血ＡＬＴ、ＡＳＴ、ＬＤＨ，并取肝组织作光镜及电镜观察。结果：再灌注９０ｍｉｎ时Ｉ／Ｒ＋Ｓｈｅｎｍａｉ组的肝组织ＭＤＡ生成，ＳＯＤ消耗，血清ＡＬＴ、ＡＳＴ、ＬＤＨ升高值均少于Ｉ／Ｒ组（Ｐ＜０．０１），且Ｉ／Ｒ＋Ｓｈｅｎｍａｉ组的肝细胞显微、超微结构损害的改变较Ｉ／Ｒ组轻。结论：参麦注射液能清除肝缺血再灌注过程中产生的氧自由基，对鼠肝缺血再灌注所致肝细胞的结构和功能损伤有保护作用

Preventive Effect of Shenmai Injection on the Damage of Ischemia Reperfusion in Rat Liver

Objective:To study the preventive effect of damage of ischemia reperfusion in rat liver by applying Shenmai Injection.Methods:37 rats were put randomly into Sham operated control(SOC) group,ischemia reperfusion(I/R) group and ischemia reperfusion Shen mai(I/R Shenmai)group.The animal model of hepatic ischemia reperfusion is made by temporarily blocking the hepatic portal blood vessels and relea sing of the hepatic portal occlusion was carried out 30 minutes later.The malondialdehyde (MDA),superoxide dismutase(SOD) in liver tissue,and AST,ALT,LDH levels in the blood were determined after the liver was reperfused for 90 minutes,the hepatic tissue was examined under light and electronic microscopies.Results:Increase of MDA,SLT,AST,LDH and decrease of SOD of I/R group were found significantly as compared with those in the I/R Shenmai group (P<0.01) .The histologic changes of the liver tissue under light and electronic microscopies in the I/R group was more obvious than in the I/R Shenmai group.Conclusion:Shenmai has the ability to scavenge oxygen free radical (OFR) and to protect from the damage of ischemia reperfusion in rat liver.