大肠癌病人肿瘤、癌旁粘膜中胃泌素、生长抑素及其分泌细胞的变化

通过观察大肠癌病人肿瘤和癌旁粘膜中胃泌素、生长抑素(SS)及其分泌细胞的变化,探讨病人体内胃泌素、SS变化的原因和意义。方法:采用放射免疫法(RIA)测定26例大肠癌病人肿瘤和癌旁粘膜中胃泌素、SS水平,并对其分泌细胞行免疫细胞化学观察。结果:肿瘤和癌旁粘膜中胃泌素含量极低且无胃泌素细胞存在。癌远旁粘膜(CDM,距肿瘤约5cm)SS水平低于癌近旁粘膜(CAM,距肿瘤0～2cm),高于肿瘤,两两相比相差均非常显著(P<0.01)。肿瘤中未见SS细胞;癌旁粘膜中SS细胞的形态、位置近于正常。CAM中SS水平与SS细胞数间呈非常显著之正相关(P<0.01)。结论:大肠癌与胃泌素、SS间存在着肯定的联系;CAM中SS上升的主要原因是粘膜中SS细胞数量增多并分泌大量的SS。这可能有助于延缓肿瘤的发生、发展,是机体对病灶的一种局部防御反应。

Change of Gastrin, Somatostatin and Their Secretory Cells in Tumor and Its Surrounding Mucosa in Colorectal Cancer Patients

Aims:To explore the reason and significance of the variation of gastrin and somatostatin (SS) in colorectal cancer patients through observing the changes of gastrin, SS and their secretory cells in tumor and its surrounding mucosa. Methods: The gastrin, SS levels in tumor and its surrounding mucosa were determined by RIA, and the gastrin, SS secretory cells in these tissues were observed by immunocytochemistry in 26 colorectal cancer patients. Results: Gastrin level in tumor and its surrounding mucosa was too low to be detected and there were no gastrin cells in those tissues. The mean SS level in cancer-distant mucosa(CDM, about 5cm from the tumor) was very significantly lower than that in cancer-adjacent mucosa(CAM, 0~2cm from the tumor), and higher than that in tumor (P< 0.01). SS cells were not found in tumor. The form and location of SS cells in tumor surrounding mucosa were similar to the normal. In CAM, there was a very significant positive correlation between the SS levels and SS cell numbers (P<0.01). Conclusions: There is a definite relationship of gastrin or SS to colorectal cancer. The elevation of SS level in CAM is mainly caused by the increase of SS cell number, which secrete more SS. The change of SS in colorectal cancer surrounding mucosa may be play an important role in inhibiting the development of the tumor, and is a local defensive reaction in the body.