探讨ELISA和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体的检测

目的:比较ELISA法和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体的检测。方法:用ELISA法与化学发光法对病人血清和标准物质进行以上三种抗体的检测,并对结果进行分析。结果:(1)化学发光法测定值较低的阳性标本,ELISA法测定有可能为阴性。(2)用化学发光法检测病人血清中HIV-1/HIV-2抗体为阴性标本的测定数据有一定程度的波动。(3)同一公司生产的以上三种抗体的标准物质,用ELISA均为阳性,可是用化学发光法仅丙肝抗体检测为阳性,而其它两种均为阴性。结论:基本证实了化学发光法比ELISA法敏感性高。

Detection of serum HIV-1/HIV-2 antibody,syphilis antibody and hepatitis C antibody by ELISA and chemiluminescence assay

Objective: To compare the ELISA and chemiluminescence assay in test of serum HIV-1/HIV-2 antibody,anti-hepatitis C antibodies and syphilis antibody.Methods: The above three antibodies were detected in serum of patients and standard substance by ELISA and chemiluminescence assay.Then the results were analyzed.Results: 1.Samples,which were found positive with low measured value by chemiluminescence assay,may be found negative by ELISA.2.Samples,which were detected by chemiluminescence assay with serum HIV-1/HIV-2 antibodies negative may exist a certain rise and fall.3.The standard substance of the above three antibodies from the same company was positive by ELISA,while with chemiluminescence assay,hepatitis C antibody was positive but the other were both negative. Conclusion: The sensitivity of chemiluminescence assay was higher than that of ELISA.