不同级别蛹虫草子实体菌粉对免疫低下小鼠非特异性免疫功能的调节作用比较

目的比较超Ⅴ星虫草和Ⅰ星虫草子实体菌粉对地塞米松诱导的免疫低下小鼠非特异性免疫功能的调节作用。方法 50只健康♂BALB/c小鼠随机分为对照组、模型组、超Ⅴ星虫草(1 g·kg^-1)组、Ⅰ星虫草(1 g·kg^-1)组和虫草胶囊(阳性药,1 g·kg^-1)组。除对照组外,其余各组于实验d 22、23腹腔注射地塞米松20 mg·kg^-1。末次给药后24 h解剖,测血常规,计算脏器指数,分析NK细胞和腹腔巨噬细胞功能及细胞因子分泌水平。结果地塞米松会导致小鼠体质量明显下降,脏器指数降低以及免疫细胞功能降低等免疫低下状态。超Ⅴ星虫草和Ⅰ星虫草均可明显拮抗地塞米松导致的免疫功能低下,在提高脾脏指数,增强NK细胞杀伤能力,提高巨噬细胞分泌TNF-α水平方面,超Ⅴ星虫草作用明显优于Ⅰ星虫草。结论超Ⅴ星虫草对地塞米松诱导的免疫功能低下的各个环节的改善作用均明显优于Ⅰ星虫草。

Comparison of effects of different levels of Cordyceps militaris power on non-specific immune function of immunodeficient mice

Aim To compare the immunomodulatory effects of Super V Cordyceps Militaris (Super V CM) and I star Cordyceps Militaris (I star CM) powder on the immune suppression induced by dexamethasone in mice.Methods Fifty male BALB/c mice were randomly divided into five groups:control and model group,Super V CM (1 g·kg^-1 ) group,I star CM (1 g·kg^-1 ) group and Cordyceps capsule(1 g·kg^-1 ) group.Apart from control group,mice of the remaining groups were injected with dexamethasone 20 mg·kg^-1 on 22nd and 23rd day of the experiment.Dissection was performed 24h after the last administration,then blood routine and viscera index,the function of NK cells and abdominal macrophages and the cytokine secretion levels were analyzed.Results Dexamethasone could significantly decrease the body mass,organ index and cellular function of immune cells in mice.The Super V CM and I star CM had significant improvement in all aspects of the immunodeficiency induced by dexamethasone.Super V CM was significantly superior to I star CM in terms of improving the spleen index,enhancing the killing ability of NK cells,and improving the secretion level of TNF-a by macrophages.Conclusion The effect of Super V CM is better than that of I star CM in enhancing non-specific immunity.