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| Lucy-tag促进海七鳃鳗嗅觉受体细胞膜表达 | |
| 引用本文： | 高翔,张哲,李伟明,任建峰,祖尧,张庆华. Lucy-tag促进海七鳃鳗嗅觉受体细胞膜表达[J]. 中国药理学通报, 2019, 0(5): 727-732 |
| 作者姓名： | 高翔 张哲 李伟明 任建峰 祖尧 张庆华 |
| 作者单位： | 1.上海海洋大学水产种质资源发掘与利用教育部重点实验室;2.上海海洋大学海洋生物科学国际联合研究中心;3.上海海洋大学国家水生动物病原库;4.密歇根州立大学渔业与野生生物系 |
| 基金项目： | 中美海洋研究中心基金资助项目(No A1-0209-15-0806);上海市教委水产高峰学科资助项目 |
| 摘 要： | 目的阐明一种多肽标签Lucy-tag对于海七鳃鳗(Petromyzon marinus)嗅觉受体膜表达的促进作用。方法用免疫细胞化学、双荧光素酶报告基因(Luciferase)系统和钙流检测法,验证多肽标签Lucy-tag对于海七鳃鳗嗅觉受体的作用。结果多肽标签Lucy-tag促进了20个嗅觉受体中13个嗅觉受体的膜表达,并且Lucy-tag不影响嗅觉受体的固有活性,Lucy-tag也不影响G蛋白偶联受体(G protein coupled receptor,GPCR)的IP_3信号通路。结论该技术为寻找嗅觉受体的配体提供了有利的技术手段,为进一步深入研究GPCR与配体的功能奠定了良好的理论基础。 |
| 关 键 词： | 嗅觉受体 G蛋白偶联受体 海七鳃鳗 Lucy-tag 免疫细胞化学 固有活性 |
Lucy-tag promotes expression of sea lamprey (Petromyzon marinus) olfactory receptor on cell membrane |
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| GAO Xiang,ZHANG Zhe,LI Wei-ming,REN Jian-feng,Zu Yao,ZHANG Qing-hua. Lucy-tag promotes expression of sea lamprey (Petromyzon marinus) olfactory receptor on cell membrane[J]. Chinese Pharmacological Bulletin, 2019, 0(5): 727-732 | |
| Authors: | GAO Xiang ZHANG Zhe LI Wei-ming REN Jian-feng Zu Yao ZHANG Qing-hua |
| Affiliation: | (Key Lab of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai201306,China;International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai201306,China;National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai201306,China;Dept of Fisheries and Wildlife,Michigan State University,East Lansing,MI 48824,USA) |
| Abstract: | Aim To elucidate the effect of Lucy-tag polypeptide label on the expression of olfactory receptor membrane in sea lamprey ( Petromyzon marinus ). Methods Immunocytochemistry,double luciferase system and calcium flow detection were used to verify the effect of polypeptide label Lucy-tag on olfactory receptors of sea lamprey. Results Polypeptide label Lucy-tag promoted the membrane expression of 13 olfactory receptors out of 20 olfactory receptors,and Lucy-tag did not affect the intrinsic activity of olfactory receptors,nor did it affect the IP 3 signaling pathway of G protein coupled receptor (GPCR). Conclusions It provides a favorable technique for finding the ligand of olfactory receptor and lays a good theoretical foundation for the further study of the function of the GPCR and ligand. |
| Keywords: | olfactory receptor GPCR sea lamprey Lucy-tag immunocytochemistry intrinsic activity |
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