肺癌抑癌基因-1对人骨肉瘤细胞株MG63的侵袭和转移抑制效应研究

目的观察肺癌抑癌基因-1（TSLC1）稳定转染至骨肉瘤细胞株后对其侵袭、转移及裸鼠皮下成瘤能力的影响。方法采用稳定表达TSLC1基因的人骨肉瘤细胞株M8T为研究对象,转染空载体的细胞系M8P设为对照组,MG63细胞株为空白组;Transwell法检测三组细胞株的体外侵袭和迁移能力;将细胞制成1×107细胞悬液,于裸鼠尾静脉注射,第4周开始处死裸鼠,肉眼及镜下观察肺部转移灶形成情况,肺组织石蜡包埋切片HE染色检测。将0.5 mL磷酸盐缓冲液（PBS）重悬2×107细胞皮下注射5周龄裸鼠,皮下肿瘤生长情况1周检测1次。结果 M8T细胞株体外侵袭细胞数目为（25.86±2.12）,迁移的细胞数目为（37.55±3.46）;其裸鼠皮下成瘤形成时间：于注射后第4周开始生长,较对照组和空白组细胞株成瘤时间晚,体积显著减小,体内肺转移形成时间为注射后第9周,肺部转移灶发生率为40%。结论 TSLC1基因可明显抑制人骨肉瘤细胞的侵袭和转移及皮下成瘤能力。

Inhibitory effect of TSLC1 on invasion and migration of human osteosarcoma cell line MG63

Objective To investigate the effects of TSLC1 on migration,invasion and tumorigenicity of human osteosarcoma cell lines stably expressing TSLC1.Methods MG63 cell is human osteosarcoma cell line,and M8T cell was developed by stable transfection of MG63 cell with TSLC1.Transwell was performed to assay the ability of migration and invasion of M8T.2×107 cells were injected into the tail vein of nude mice.Mice were sacrificed on the week 4 after injection,and lung metastases were evaluated under both macroscopic and microscopic observation with HE staining.1×107 cells in 0.2 mL PBS were injected into the two flanks of 5-week-old nude mice.The tumor growth was monitored once a week.Results The number of invasive and migrating TSLC1-transfected cells was significantly suppressed in vitro（25.86±2.12 and 37.55±3.46）.Moreover,tumorigenicity of M8T cells was suppressed in vivo,lung metastases and subcutaneous tumor formation of nude mice occurred later and less than control and blank groups.Conclusion The abilities of migration,invasion and tumorigenicity of M8T cells stably expressing TSLC1 were decreased obviously in vivo and in vitro.