驱虫斑鸠菊提取物抑制恶性黑色素瘤A375细胞增殖的研究

目的探讨驱虫斑鸠菊提取物对人恶性黑色素瘤A375细胞的增殖以及对其酪氨酸酶活性和黑色素含量的影响。方法四甲基噻唑氮蓝比色法测定对A375细胞抑制作用;显微法观察细胞形态;比色法测定酪氨酸酶活性和黑色素含量。结果在一定质量浓度范围(1～40mg.L-1)内随着驱虫斑鸠菊提取物质量浓度增加可抑制黑色素瘤细胞的增殖;驱虫斑鸠菊提取物质量浓度小于10mg.L-1,对酪氨酸酶活性和黑色素合成抑制有增加的趋势;大于20mg.L-1时,显示一定的细胞毒性作用。结论驱虫斑鸠菊提取物能显著抑制恶性黑色素瘤A375细胞的增殖,在一定质量浓度范围内(1～40mg.L-1)有浓度依赖关系(P<0.05)。

Effect of Vernonia anthelmintica Willd extract on inhibiting the proliferation of melanoma A375 cells

Objective To explore the effects of Vernonia anthelmintica Willd.extract on the inhibition of proliferation and to study the tyrosinase activity and melano genesis in human melanoma A375 cells.Methods The cell proliferation inhibition was measured by methyl thiazolyltet razolium(MTT)colorimetric assay.The tyrosinase activity and melanin content were measured by colorimetry assay.Results When the concentration of Vernonia anthelmintica Willd was range from 1 mg·L-1 to 40 mg·L-1,a proli-feration inhabiting effect was shown with the increase of the concentration.When the concentration was lower than 10 mg·L-1,a positive effect of tyrosinase activity and melano genesis was shown.When the concentration was above 20 mg·L-1,cytotoxic effect occurred.Conclusions There is a concentration-dependent relationship between the Vernonia anthelmintica Willd.extract and the inhibition effect of A375 cells proliferation(P<0.05).