Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells |
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| Authors: | Man-Chin Hua Tzou-Yien Lin Ming-Wei Lai Man-Shan Kong Hung-Ju Chang Chien-Chang Chen |
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| Affiliation: | Department of Pediatrics Chang Gung Memorial Hospital Keelung 204 Taiwan China Graduate Institute of Clinical Medical Sciences College of Medicine Chang Gung University Taoyuan 333 Division of Infectious Disease Division of Gastroenterology Department of Pe diatrics |
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| Abstract: | AIM:To investigate the immune response of peripheral blood mononuclear cells(PBMCs)and dendritic cells (DCs)that were stimulated by probiotic preparations. METHODS:PBMCs were isolated,cultured,and stimulated with Bio-Three(a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis;105, 10 6 and 10 7 CFU/mL for 24 h).Cytokine production of (1)circulating PBMCs;(2)PBMCs stimulated by probiotic preparation;(3)monocyte-derived DCs;and(4)DC andT cell co-culture was determined by enzyme-l... |
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| Keywords: | Probiotics Bio-Three Peripheral blood mononuclear cells Dendritic cells |
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