人胃癌组织淋巴管的超微结构及其与淋巴转移的关系

目的研究人胃癌组织淋巴管的超微结构特点,探讨胃癌淋巴转移机制。方法取人胃癌中心区、周边区及正常区组织块,常规固定,树脂包埋,半薄切片,光镜下定位,检出淋巴管经超薄切片,透射电镜观察。结果人胃癌中心区未见淋巴管;胃癌周边区较正常区淋巴管增多(t=3.270,P=0.002),管腔较小(t=-5.315,P=0.000),开放连接增多(χ2=12.895,P=0.000),淋巴管破坏增多(χ2=15.674,P=0.000);淋巴转移组胃癌周边区比无淋巴转移组胃癌周边区淋巴管多(t=2.523,P=0.021),被破坏的淋巴管多(χ2=5.281,P=0.022),开放连接/非开放连接数差异无统计学意义(χ2=0.217,P=0.641)。结论人胃癌周边区存在淋巴管新生;胃癌细胞可能是通过胃癌周边区被破坏的淋巴管进行转移的。

ULTRASTRUCTURAL CHARACTERISTICS OF LYMPHATIC VESSELS IN HUMAN GASTRIC CANCER AND ITS RELATIONSHIP WITH LYMPH NODE METASTASIS

Objective To study the ultrastructural features of lymphatic vessels in human gastric carcinoma and discuss the metastasis mechanism of malignant cells by lypmphatic vessels. Methods The central, peripheral and normal regions of gastric cancer were obtained from postoperative samples of patients. Common resin embedded specimens were made. Lymphatic vessels were localized by hemithin sections under light microscope and observed by ultrathin sections under transmission electron microscope.Results There was no lymph vessel in carcinomatous central region. Compared with the normal region, the peripheral region of gastric carcinoma was found with more lymphatic vessels(t=3.270,P=0.002),more destroyed lymphatic vessels (χ SUP>2/SUP>=15.674,P=0.000),more interendothelial openning junctions（χSUP>2/SUP>=12.895,P=0.000）,and smaller lumina (t=-5315，P=0.000). Compared with gastric carcinoma without lymph node metastasis, there were more lymphatic vessels in peripheral region of gastric carcinoma with lymph metastasis（t=2.523，P=0.021）, more destroyed lymphatic vessels（χSUP>2/SUP>=5.281，P=0.022), and there was no statistical significance in the numbers of interendothelial opening and non opening junctions （χ SUP>2/S