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The LDL receptor-related protein can form homo-dimers in neuronal cells |
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| Authors: | Alexandra Makarova Kathryn K. Bercury Kenneth W. Adams Daniel Joyner Meihua Deng Robert Spoelgen Mirjam Koker Dudley K. Strickland Bradley T. Hyman |
| Affiliation: | 1. MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA;2. Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD 21201, USA |
| Abstract: | The ability of the low density lipoprotein receptor-related protein (LRP) to form homo-dimers was studied in mouse neuroblastoma and human neuroglioma cells as well as in primary cortical cultures from adult mouse brain. Homo-dimerization of LRP light chain (LC) was shown by several methods including co-immunoprecipitation, fluorescence lifetime imaging microscopy, and bimolecular fluorescence complementation assay. The requirement of intact NPXY motifs of LRP LC for homo-dimerization was ruled out by co-immunoprecipitation assay. |
| Keywords: | LRP Low density lipoprotein receptor-related protein Dimer Homo-dimerization |
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