乌龙茶及其有效成分对肝癌细胞HepG2的生长抑制作用

目的 通过探讨茶及其有效成分对肝癌细胞HepG2增殖的影响,为乌龙茶成分进一步开发提供有力的实验室证据.方法 体外培养人肝癌细胞株HepG2,加入浓度为25、50、100和200 mg/L的茶叶浸出液、茶多酚、茶色素、茶多糖、茶氨酸作用24h后,用水溶性四唑盐(WST-1)检测肝癌细胞的存活率.结果 乌龙茶及其有效成分对肝癌细胞HepG2进行干预后,各组肝癌细胞的增殖均被抑制,各组的吸光度值均低于阴性对照组,茶汤100 mg/L组和200 mg/L组、茶多酚各组和茶色素各组、茶多糖和茶氨酸50mg/L、100 mg/L和200 mg/L组,与阴性对照组比较,差异均有统计学意义(均P＜0.05).同时茶多酚25 mg/L组、茶色素25 mg/L组与茶汤25 mg/L组比较,差异均有统计学意义[(0.1658±0.0025)、(0.1913±0.0013)比(0.2005±0.0013),均P＜0.05]；茶多酚50mg/L组、茶色素50mg/L组、茶多糖50 mg/L组、茶氨酸50 mg/L组与茶汤50 mg/L组比较,差异均有统计学意义[(0.1545±0.0041)、(0.1655±0.0039)、(0.1595±0.0019)、(0.1728 ±0.0040)比(0.1970 ±0.0078),均P＜0.05]；茶多酚100 mg/L组(0.1370±0.0016)与茶汤100 mg/L组(0.1600 ±0.0037)比较,差异有统计学意义(P＜0.05).结论 乌龙茶及其有效成分对人肝癌细胞HepG2具有明显的抑制作用,表现出抗肝癌的作用.

The effect of oolong tea ingredients on liver cancer

Objective To study the effect of oolong tea and tea ingredients on liver cancer. Methods The human hepatoma cell line G2 （HepG2）was incubated in RPMI1640. HepG2 was exposed to oolong tea and tea polyphenol ,tea pigment, theanine and tea polysaceharide at the different concentrations of 25 mg/L,50 mg/L, 100 mg/L and 200 mg./L for 24 hours, respectively. The growth inhibition of HepG2 cells was measured by WST-1 assay. Results The concentrations of tea in the 100 mg,/L and 200mg/L groups were significantly lower than those in control group（ P 〈 0. 05 ）. The concentrations of tea polyphenol, tea pigment in 25 mg,/L,50 rag/L, 100 mg/L and 200 mg/L groups were significantly lower than control group（0. 2017 ± 0. 0018 ）（ P 〈 0. 05 ）. The concentrations of theanine and tea polysaccharide in the 50 mg/L（0. 1595 ± 0. 0019,0. 1728 ± 0. 0040）, 100 rag/L（0. 1453 ± 0. 0017, 0. 1555 ± 0. 0045 ）and 200 mg/L groups （0. 1305 ± 0. 0024,0. 1405 ± 0. 0013 ）were significantly lower than those in control group（P 〈 0. 05）. The concentrations of tea polyphenol （0. 1658 ± 0. 0025 ）and tea pigment （0. 1913 ± 0. 0013 ）groups were significantly lower than those in tea group in the 25 mg/L（0. 2005±0. 0013） （P 〈0.05）. The concentrations of tea polyphenol（0. 1545 ± 0. 0041 ）, tea pigment （0. 1655 ± 0. 0039 ）, theanine （0. 1595 ± 0. 0019 ） and tea polysaccharide （0. 1728 ± 0. 0040） were significantly lower than those in tea group（ 0. 1970 ± 0. 0078）in the 50 mg/L（ P 〈 0. 05 ）. The concentrations of tea polyphenol （0. 137 ± 0. 0016 ） were significantly lower than those of tea group in the 100 mg/L（0. 160 ± 0. 0037 ） （ P 〈 0. 05 ）. Conclusions The results indicate that oolong tea and tea ingredients can inhibit the proliferation on HepG2 cells. It is suggested that oolong tea and tea ingredients can be used to treat human liver carcinoma.