BRMS1在不同转移性乳腺癌细胞中的表达及其与HDAC活性的关系

目的:探讨肿瘤转移抑制基因（BRMS1）在不同转移性乳腺癌细胞中的表达及其与组蛋白去乙酰化酶（HDAC）活性的关系和意义。方法:采用 RT-PCR技术检测3种细胞株〔非转移性MCF-7(A株)，低转移性MDA-MB-453（B株），高转移性MDA-MB-231（C株）〕中BRMS1基因的表达。用Western blot检测在上述3种细胞株中BRMS1蛋白的表达。ELISA检测3种细胞株中HDAC的活性。结果:(1) 在A，B，C 3种细胞株中BRMS1 基因表达依次比例为3.1∶2.0∶1.0。C细胞株比A细胞株BRMS1基因表达降低2.1倍，BRMS1 基因表达随转移程度的增高而明显降低（P＜0. 001)。(2)在A，B，C 3种细胞株中BRMS1蛋白表达依次比例为3.2∶1.9∶1.0。C细胞株比A细胞株BRMS1蛋白表达降低2.2倍，BRMS1随转移程度的增高而明显降低（P＜0. 001)。(3)A，B，C 3种细胞株中HDAC活性依次比例为1.0∶1. 8∶2.6。C细胞株比A细胞株HDAC活性增高1.6倍，随转移程度的增高HDAC活性升高（P＜0. 001)。结论:（1）BRMS1基因和蛋白的表达在3种细胞株中随转移程度的增高而呈递减趋势。（2）HDAC活性在3种细胞株中随转移潜能的增高而呈递增趋势;提示BRMS1可能通过参与调节HDAC活性调控乳腺癌的转移。

Expression of BRMSI in different metastasic breast cancer cells and its relation to HDAC activity

Abstract:Objective:To investigate the expression of BRMS1 in different metastatic breast cancer cells and its relation to HDAC activity. Methods :The gene expression of BRMS1 in 3 types breast cancer cell lines〔non-metastasis MCF-7（A cell line）, low metastasis MDA-MB-453（B cell line）and high metastasis MDA-MB-231（C cell line）〕 was determied by RT-PCR technique; the protein expression of BRMS1 was measured with Western blot technique and HDAC activity by enzyme linked immunosorbent assay.Results:（1） Gene expressive ratio of BRMS1 was 3.1∶2.0∶1.0 in the A、B、C cell lines, respectively. The gene expression of BRMS1 in the C cell line decreased 210% compared to A cell line, and expression of BRMS1 was markedly reduced as the degree of metastasis increased (P＜0.001). （2）protein expression ratio of BRMS1 was 3.2∶1.9∶1.0 in the A、B、C cell lines. The protein expression of BRMS1 in the C cell line decreased by 220% compared to A cell line, and protein expressive of BRMS1 was reduced in relation to metastasis degree of breast cancer cell line (P＜0.001). （3）HDAC activity ratio was 1.0∶1. 8∶2.6 in the A、B、C cell lines . HDAC activity in the C cell line increased 160% compared to A cell line,HDAC activity increased in relation to metastasis degree of the cell line (P＜0.001).Conclusions:（1）Gene and protein expression of BRMS1 is reduced according to the metastasis degree increasing of the cell line. （2）HDAC activity increases with increase of degree of metastasis of the 3 cell types，and suggests that BRMS1 may regulate breast cancer metastasis via HDAC activity.