山莨菪碱对大鼠肝脏缺血再灌注损伤保护作用的实验研究

目的 探索山莨菪碱对大鼠肝脏缺血再灌注损伤的保护作用。方法 选雄性Wistar大鼠160只，分为正常对照组、缺血再灌注组、生理盐水组和山莨菪碱组，观察了肝脏缺血60分钟及再灌注1、3、6、12及24小时后血浆内皮素－1（ET－1）、透明质酸（HA）和谷丙转氨酶（ALT）含量变化及肝组织病理学变化。结果 肝脏缺血再灌注后，血浆ET－1、HA和ALT含量均明显增高，同时肝脏瘀血很明显，肝脏缺血再灌注前用山莨菪碱后，血浆HA和ALT含量明显降低，同时肝组织瘀血减轻。结论 山莨菪碱可改善再灌注后的肝脏微循环障碍，对大鼠肝脏缺血再灌注损伤有保护作用。

EXPERIMENTAL STUDY ON PROTECTIVE EFFECTS OF ANISODAMINE ON ISCHEMIA-REPERFUSION INJURY IN RAT LIVER

Objective To study the protective effects of anisodamine on liver ischemia reperfusion injury in rats. Methods One hundred and sixty male Wistar rats were randomly divided into the normal control ( n= 10), ischemia reperfusion ( n =50), normal saline ( n =50) and anisodamine ( n =50, 2.0 mg/kg) groups. The animals were killed 1, 3, 6, 12, 24 hours after ischemia induced for 60 minutes and followed by reperfusion. Plasma endothelin 1 (ET 1), hyaluronic acid (HA), glutamic pyruvic transaminase enzyme (ALT) were measured, and the hepatic histopathologic alterations were also observed. Results The plasma ET 1, HA and ALT concentrations were markedly increased after liver ischemia reperfusion.The hepatic congestion was significantly obvious. An intravenous injection of anisodamine before ischemia reperfusion could decrease the plasma HA and ALT concentrations and relieve the hepatic congestion. Conclusion Anisodamine can improve hepatic microcirculatory disturbances after reperfusion and have hepatoprotictive effects on rat liver ischemia reperfusion injury.