| 冬凌草甲素对人膀胱癌细胞株BIU-87的生长抑制作用及机制探讨 |
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| 引用本文： | 车宪平,韩瑞发,肖劲逐,张国庆,陈道虎,徐磊. 冬凌草甲素对人膀胱癌细胞株BIU-87的生长抑制作用及机制探讨[J]. 山东医药, 2009, 49(46): 4-6 |
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| 作者姓名： | 车宪平 韩瑞发 肖劲逐 张国庆 陈道虎 徐磊 |
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| 作者单位： | 海南省农垦总局医院,海口,570311;天津市泌尿外科研究所 |
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| 基金项目： | 海南省卫生厅2008年度科研基金资助项目 |
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| 摘 要： | 目的 观察冬凌草甲素(ORI)对膀胱癌细胞BIU-87的生长抑制作用并探讨其作用机制.方法 用MTT法检测ORI作用后BIU-87的生长抑制情况;用光学显微镜和电子显微镜观察细胞形态;用流式细胞仪检测细胞凋亡;分光光度法检测BIU-87中Caspase-3的活性变化;用端粒酶重复扩增(TRAP)-PCR-银染法检测其端粒酶活性的变化.结果 ORI对BIU-87有明显的生长抑制作用,呈时间-效应关系和浓度-效应关系.随着ORI作用时间延长,BIU-87凋亡增加,BIU-87内的Caspase-3活性增加,端粒酶活性逐渐降低.结论 ORI对BIU-87有明显的生长抑制作用;其机制可能是通过活化细胞内的Caspase-3和抑制端粒酶活性诱发BIU-87细胞凋亡的.
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| 关 键 词： | 冬凌草甲素 膀胱肿瘤 BIU-87细胞 细胞凋亡 |
Inhibition effect and mechanism of oridonin on BIU-87 cells |
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| CHE Xian-ping,HAN Rui-fa,XIAO Jin-zhu,ZHANG Guo-qing,CHEN Dao-hu,XU Lei. Inhibition effect and mechanism of oridonin on BIU-87 cells[J]. Shandong Medical Journal, 2009, 49(46): 4-6 |
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| Authors: | CHE Xian-ping HAN Rui-fa XIAO Jin-zhu ZHANG Guo-qing CHEN Dao-hu XU Lei |
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| Affiliation: | CHE Xian-ping1,HAN Rui-fa,XIAO Jin-zhu,ZHANG Guo-qing,CHEN Dao-hu,XU Lei(1 Hainan Provincial Nongken Hospital,Haikou 570311,P.R.China) |
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| Abstract: | Objective To observe the antineoplastic effect of oridonin on bladder cancer BIU-87 cell lines in vitro and explore its mechanisms.Methods The inhibitory rate of the cells was measured by MTT assay.Morphology of cell apoptosis was observed by light and electron microscopy.Cell apoptotic rate was detected by flow cytometry(FCM).Caspase-3 was assayed by colorimetric assay kit.Telemerase activity was detected by telemerase repeat amplification proctocol(TRAP)-polymerase chain reaction(PCR)-silver stainning.Res... |
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| Keywords: | oridonin bladder neoplasm BIU-87 cells apoptosis |
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