HPLC法测定吡嘧司特钾滴眼液的含量

目的建立高效液相色谱法对吡嘧司特钾滴眼液的含量测定方法。方法采用反相高效液相色谱法。色谱条件:Agilent Zorbax SB-Aq C18柱,流动相为:甲醇-水(用冰醋酸调pH值为3.5)(40∶60),检测波长为357nm,流速为1.0mL.min-1。结果在选定的色谱条件下吡嘧司特钾滴眼液中辅料对主药无干扰,样品在1~50μg.mL-1浓度范围内,峰面积与浓度线性关系良好,相关系数r=0.9999,平均回收率为99.96%,RSD为0.2%。结论本方法简便,准确,专属性强,可用于吡嘧司特钾滴眼液的含量测定。

Determination of Pemirolast Potassium Eye Drops by HPLC

OBJECTIVE To establish an HPLC method for the determination of Pemirolast Potassium Eye Drops.METHODS Analysised by RP-HPLC,an agilent Zorbax SB-Aq C18 column was used,a mixture of methanol-water(ajusted the pH to 3.5 by acetic acid)(40∶60)was used as the mobile phase.The flow rate was 1.0mL·min-1 and the detection wavelength was 357nm.RESULTS At the validated method,the stability tests under various conditons were performed.The standard curve was liner over the range of 1~50μg·mL-1 with the correlation codfficient of 0.9999.The average recovery was 99.6%.CONCLUSION This method was sample,accurate,specific and could be used in quality control of Pemirolast Potassium Eye Drops preparations.