脑出血患者载脂蛋白E基因型分布及相关研究

目的研究脑出血患者载脂蛋白E(apoE)基因多态性分布特点,探讨脑出血患者apoE基因型与神经功能缺损和血清高敏C反应蛋白(hs-CRP)水平的关系。方法选择脑出血患者78例(脑出血组)和健康体检者91例(对照组),利用PCR-RFLP技术,测定apoE基因型;采用生化全自动仪分别对TC、TG、LDL-C、HDL-C、空腹血糖和血清hs-CRP水平等进行测定;脑出血患者入院时采用美国国立卫生研究院卒中量表(NIHSS)进行神经功能缺损评分。结果脑出血组apoEε4基因频率明显高于对照组,apoEε2基因频率明显低于对照组(P<0.05,P<0.01);脑出血组TC、TG、LDL-C、空腹血糖水平明显高于对照组,HDL-C水平明显低于对照组(P<0.05);脑出血组apoEε4携带者NIHSS评分明显高于非apoEε4携带者(P<0.05);脑出血组apoEε2和apoEε4携带者血清hs-CRP水平明显高于非apoEε2和非apoEε4携带者(P<0.05)。结论 apoEε4可能是脑出血的危险因素;脑出血急性期炎性反应可能与apoEε2和apoEε4有关。

Apolipoprotein E polymorphism in cerebral hemorrhage patients

Objective To study the distribution characteristics of apolipoprotein E(apoE) polymorphism and the relation of apoE genotype with neurologic defect and serum high sensitivity C-reactive protein(hs-CRP) level in cerebral hemorrhage patients.Methods Seventy-eight cerebral hemorrhage patients served as a cerebral hemorrhage group and ninety-one subjects undergoing health check served as a control group in this study.Their apoE genotype was detected by polymerase chain reaction-restriction fragment length polymorphism,their serum levels of TC,TG,HDL-C,LDL-C,blood glucose and hs-CRP were measured with an automatic biochemical analyzer,and their neurologic defect was scored at admission according to the national institutes of health stroke scale.Results The frequency of apoEε4 genotype and the serum TC,TG,LDL-C and blood glucose levels were significantly higher while the frequency of apoEε2 genotype and serum HDL-C level were significantly lower in cerebral hemorrhage group than in control group(P<0.05,P<0.01).The national institutes of health stroke scale score was significantly higher in cerebral hemorrhage group with apoEε4 than in that without apoEε4(P<0.05).The serum hs-CRP level was significantly higher in cerebral hemorrhage group with apoEε2 and apoEε4 than in that without apoEε2 and apoEε4(P<0.05).Conclusion apoEε4 may be the risk factor for cerebral hemorrhage and inflammatory reaction in acute cerebral hemorrhage may be related with apoEε2 and apoEε4.