复方鳖甲方治疗实验大鼠特发性肺纤维化的超微结构观察

目的研究复方鳖甲方治疗博莱霉素诱导的实验大鼠特发性肺纤维化(Id iopathic pulmonaryfibrosis IPF)的超微结构变化。方法180只SD雄性大鼠随机分为6组,假手术对照组、模型组、阳性药物对照组及复方鳖甲方高、中、低剂量组。在药物治疗的第7、14、28天,每次每组随机抽取大鼠10只麻醉后取材,常规固定制成切片,光镜观察其结构和胶原变化。通过图像分析系统,测定支气管周围及肺泡透光区、致密区的平均光密度。常规制造电镜切片观察其超微结构。结果光镜观察显示复方鳖甲方可降低肺纤维化程度。图像分析表明:假手术组和模型组在7、14、28 d之间光密度无显著性差异;其余各组三阶段之间有不同差异。电镜观察,复方鳖甲方各组在7、14、28 d胶原纤维含量均明显少于模型组和阳性药物组,基膜区域性折叠、扭曲或缺损。细胞外基质沉积、呼吸膜增厚等现象也明显好于模型组和阳性药物组。结论复方鳖甲方有明显抑制实验大鼠IPF胶原纤维增生的作用。

Observation on ultrastructure in rats with IPF treated with Fufangbiejia Formula

Objective To study the ultrastructure changes in rats with idiopathic pulmonary fibrosis(IPF) induced by bleomycin treated with Fufangbiejia Formula.Methods To divide randomly 180 male SD rats into the sham-operation group(group1),model group(group2),positive control group(group3),groups with high,medium and low dose of Fufangbiejia Formula(group4,5 and 6).On 7~(th),14~(th)and 28~(th) day of the treatment 10 rats every time in every group were randomly selected,anesthelized and sliced up,and the changes of structure and collagen were observed by the light(microscope).The(average) optical density around the bronchi,alveolar nonopaque and compact zones were detected by the(imagine) system.The ultrastructures of the slices were observed by the electron(microscope).Results The result of the light microscope showed that Fufangbiejia Formula relieved the(degree) of pulmonary fibrosis.The imagine analysis showed that there was no significant difference(between) group1 and group2 on 7~(th),14~(th) and 28~(th) day,while there were differences between other groups.The result of the electron(microscope) showed that the content of collagen fibers in group4,5 and 6 were significantly lower than that of group2 and 3 on 7~(th),14~(th) and 28~(th) day.The regional fold,torsion or defection of inophragma,sludging of extracorpuscular base,and the thickening of respiratory tract membrane were less serious in group4,5 and 6 than that in group2 and 3.Conclusion Fufangbiejia Formula can inhibit obviously the accrementition of collagen fibers in rats with idiopathic pulmonary fibrosis.