交互移动窗口因子方法在桂枝-茯苓配伍前后挥发油组分及溶出率变化研究中的应用

目的：利用气相色谱-质谱(GC-MS)对药对桂枝-茯苓及单味药桂枝的挥发油进行分析。方法：采用色谱柱HP-5MS(250 μm×30 m，0.25 μm)分离，起始温度60 ℃,维持2 min,以4 ℃·min^-1升至130 ℃,再以1 ℃·min^-1升至160 ℃,维持2 min,再以4 ℃·min^-1升至240 ℃,维持20 min，分流比1∶30，进样量1 μL，结合新近提出的化学计量学分辨方法-交互移动窗口因子分析方法及Kovats保留指数对两者挥发性成分中的共有组分、差异组分及各成分溶出率进行比较分析。结果：分别定性了42和46种组分。结论：研究显示两者挥发性成分种类基本保持不变，但各成分溶出率均有显著变化：少部分组分溶出率明显增加，大部分组分溶出率显著下降，甚至有个别组分消失。研究表明药对桂枝-茯苓的药效物质基础不仅是单味药有效成分的简单加和，还应考虑单味药在配伍过程中发生的物理化学变化对药效的影响，具有较强的临床参考价值。

AMWFA method applied to research on changes of essential components andextractive ratioes of herbal pair Cinnamomum cassia and Poria cocos

Gas chromatography/mass spectrometry, chemometric resolution method-Alternative movingwindow factor analysis that were proposed recently and the Kovats retention index were used to analyze the essential components of herbal pair Cinnamomum cassia Presl and Poria cocos (CCP-PC) and compare them with those of single herbal Cinnamomum cassia Presl (CCP). 46 and 42 essential components in essential oil of CCP and CCP-PC have been identified individually. Results shows that the number of essential components of CCP and CCP-PC were almost the same, but extractive ratioes of them have changed significantly, some of them were increased obviously, most of them were declined notably instead and even several ingredients of CCP-PC were disappeared due to CCP PC's interaction probaloly. The main pharmacodynamic ingredients of CCP-PC, 3-phenyl-2-propenal and Cinnamaldehyde were obviously higher contents than that of single CCP. It suggested that there exist certain interactions of the chemical ingredients in compound medicine rather than their sum effect of single medicines. There is a litter difference in quality and quantity between single medicines and pair medicines, so the application of pair medieines can expand single medicine's adaptive disease and has a good clinical referenced valuation.