| 新型表皮细胞生物膜移植物的研究 |
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| 引用本文： | 焦向阳,邢新,Kopp J,Kopp J. 新型表皮细胞生物膜移植物的研究[J]. 中国修复重建外科杂志, 1999, 13(2): 105-108 |
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| 作者姓名： | 焦向阳 邢新 Kopp J Kopp J |
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| 作者单位： | 1. 第二军医大学附属长海医院整形外科,上海,200433
2. Department of Plastic and Hand Surgery, Freiburg University, Germany |
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| 摘 要： | 目的将人表皮细胞接种到生物膜上构建一种新的表皮替代物———表皮细胞生物膜移植物（ＭＣＧ）。方法新鲜和冷冻保存的ＭＣＧ移植到裸鼠全厚皮肤缺损创面,以单纯无接种细胞的生物膜作为对照。并行组织学、免疫组织化学及电镜观察。结果表皮细胞可以生物膜为载体在体外进行培养,达６０％～７０％亚饱和状态时,表皮细胞生物膜可一起移植到创面,在创面上表皮细胞继续增殖分化,形成一层新生表皮。结论表皮细胞生物膜移植物可作为一种新型表皮替代物修复皮肤缺损
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| 关 键 词： | 表皮细胞 生物膜 生物膜细胞移植物 创面愈合 |
THE STUDY OF NEW BIO-MEMBRANE-BRED EPIDERMAL CELL GRAFTS |
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| JIAO Xiang-yang,XING Xin,Kopp J,Kopp J. THE STUDY OF NEW BIO-MEMBRANE-BRED EPIDERMAL CELL GRAFTS[J]. Chinese journal of reparative and reconstructive surgery, 1999, 13(2): 105-108 |
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| Authors: | JIAO Xiang-yang XING Xin Kopp J Kopp J |
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| Affiliation: | Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200443. jscao@ecmu.org.cn |
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| Abstract: | Objective The human epidermal cells were bred on a kind of bio membrane, the bio brane, in engineering a kind of new epidermal substitute, the bio membrane bred cell graft. Methods Fresh and frozen grafts of biomembrane bred epidermal cells were transplanted into the full thickness wounds of nude mice and those received simple Bio brane were served as control. The wounds of the two groups were observed daily and biopsy was taken on the 3,5,7,10,21 and 35 days respectively. Results Epidermal cells could be cultured in vitro on the bio membrane reaching the sub saturated state of 60 to 70 percents. The bio membrane after being grafted the epidermal cells continued to proliferate and differentiate to form a layer of new epidermis. There was no difference between the fresh and the frozen bio membranes.Conclusion Bio membrane bred with epidermal cells could be a kind of ideal epidermal substitute. |
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| Keywords: | Epidermal cell Bio membrane Membrane bred epidermal cell graft Wound healing |
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