小C肽人胰岛素原类似物(B-Arg-Arg-A)和人胰岛素原(B-C-A)A、B链重组条件的研究

目的 :获得更佳的变复性条件和提高人胰岛素原的重组率。方法 :采用特有的重组方法 ,将小C肽人胰岛素原类似物 (B Arg Arg A)与人胰岛素原 (B C A)进行重组条件的比较。 结果 :发现二者在 pH10 .6左右处重组率达到最大 ,且在相同的条件下B Arg Arg A蛋白质的重组率 (94% )较B C A蛋白质 (82 % )的高。结论 :进一步验证了“胰岛素的A、B链含有足够使二硫键正确配对的结构信息”的论点。

Study on the Recombining Conditions Between A and B Chains of "Mini-C" Human Proinsulin Analog and Human Proinsulin

Purpose:The aim is to obtain the best conditions of reduction and oxidization and to raise the recombinating rate of human Proinsulin.Methods:“Mini C” human Proinsulin Analog(B Arg Arg A) was compared with human Proinsulin(B C A) by specifical methods of recombination.Results:It showed that the recombination ratio of both proteins reached a maximum in pH 10.6.However under the same conditions, the recobination ratio of B-Arg-Arg-A(94%) was higher than that of B C-A(82%).Conclusion:These experiments also proved that A and B chains of insulin involve enough structural information about the correct pairing of the disulfide bonds.