| 人肝癌特异性EB病毒载体—p^EBAF介导hGM—CSF基因的转移及表达 |
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| 引用本文： | 李玉富,张俊萍,孙声桃,李继昌,李福胜,张智清. 人肝癌特异性EB病毒载体—p^EBAF介导hGM—CSF基因的转移及表达[J]. 中国现代医学杂志, 2001, 11(3): 17-19 |
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| 作者姓名： | 李玉富 张俊萍 孙声桃 李继昌 李福胜 张智清 |
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| 作者单位： | 1. 河南省肿瘤医院内科
2. 河南医科大学 |
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| 基金项目： | 本课题为"863"高科技计划资助项目(83-102-11-09) |
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| 摘 要： | 目的：探索人粒细胞－巨噬细胞集落刺激因子（hGM-CSF)基因在肝癌细胞中特异性表达的新途径。方法：构建p^EBAF/hGM-CSF基因克隆，用脂质体转染法将它分别导入分泌甲胎蛋白（AFP）的肝癌细胞株HepG2和不分泌APF的细胞株SMMC7721，构建两围基因的细胞克隆HepG2/hGM-CSF,SMMC7721/hGM-CSF,用MTT比色法测定细胞上清中hGM-CSF活性。结果：HepG2/hGM-CSF细胞上清hGM-CSF活性平均为46．5ng/ml/10^6/24h，SMMC7721细胞上清中无明显hGM-CSF活性。结：载体p^EBAF介导的hGM-CSF基因能在分泌AFP的肝细胞中获得特异性表达。
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| 关 键 词： | EB病毒表达载体 人粒细胞－巨噬细胞集落刺激因子 基因治疗 肝癌 |
| 修稿时间： | 2000-12-20 |
hGM-CSF GENE TRANSFERMEDIATED BY HUMANHEPATOCELLULAR CARCINOMA (HCC) TISSUE SPECIFIC EBV- BASED VECIOR pEBAFAND ITS TISSUE- SPECIFIC EXPRESSION |
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| Li Yufu,Zhang Junping,Sun Shengtal,LI Jichang,Li Fusheng,Zhang Zhiqing. hGM-CSF GENE TRANSFERMEDIATED BY HUMANHEPATOCELLULAR CARCINOMA (HCC) TISSUE SPECIFIC EBV- BASED VECIOR pEBAFAND ITS TISSUE- SPECIFIC EXPRESSION[J]. China Journal of Modern Medicine, 2001, 11(3): 17-19 |
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| Authors: | Li Yufu Zhang Junping Sun Shengtal LI Jichang Li Fusheng Zhang Zhiqing |
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| Abstract: | Objective:To investigate a new way of tissue-specific expression of human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene in HCC.Methods:Construct p EBAF/hGF-CSF,transferred into HCC cell line HepG2 (secreting AFP) and SMMC7721 (not secreting AFP) by lipofectin,two positive cell lines HepG2/hGM-CSF and SMMC7721/hGM-CSF.Results:By MTT assay the cytokine biological activity revealed the HepG2/hGM-CSF expressed hGM-CSF for 46.5ng/ml/106/24hr,but SMMC7721 has no expression of hGM-CSF were established.Conclusions:hGM-CSF gene mediated by p EBAF has the ablility of tissue-specific expression in HCC cells. |
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| Keywords: | EBV-based vector hGM-CSF Gene Therapy |
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