推拿改善兔椎动脉型颈椎病血供时效性的实验研究

目的:通过建立椎动脉型颈椎病(CSA)家兔模型,观察推拿改善椎动脉血流量时效性的特点,以及基于NPY、ET-1探讨这种时效性的物质基础。方法:将50只6月龄体质量为(2.0±0.5)kg,雌雄各半的清洁级新西兰大耳白兔,采用随机数字表方法分为空白组、模型组、推拿10 min组、推拿20 min组和推拿30 min组,每组10只。除空白组外均行注射硬化剂法制备CSA家兔模型,推拿10、20、30 min组行相应时长的推拿治疗,每日1次,连续10 d;分别于治疗前和治疗后使用Peri Flux 5000激光多普勒仪检测各组家兔椎动脉血流量,采用ELISA法检测血清中NPY、ET-1含量。结果 :治疗前后椎动脉血流量变化方面比较:模型组(0.009±0.775)PU与推拿10 min组(5.117±0.712)PU比较差异无统计学意义,模型组与推拿20 min组(11.634±0.999)PU、30 min组(11.831±0.992)PU比较差异有统计学意义(P0.01),推拿10 min组与推拿20、30 min组比较差异有统计学意义(P0.01),推拿20 min组与推拿30 min组比较差异无统计学意义。治疗前后NPY含量变化方面比较模型组(-0.327±0.863)pg/ml与推拿10 min组(24.518±6.027)pg/ml比较差异有统计学意义(P0.05),模型组与推拿20 min组(46.141±8.196)pg/ml、推拿30 min组(47.091±10.699)pg/ml比较差异有统计学意义(P0.01),推拿10 min组与推拿20、30 min组比较差异有统计学意义(P0.01),而推拿20 min组与推拿30 min组比较差异无统计学意义。ET-1含量变化方面比较模型组(0.300±1.109)pg/ml与推拿10 min组(3.283±13.385)pg/ml比较差异无统计学意义,模型组与推拿20 min组(36.701±8.928)pg/ml、推拿30 min组(37.719±7.320)pg/ml比较差异有统计学意义(P0.01),推拿10 min组与推拿20、30 min组比较差异有统计学意义(P0.01),而推拿20 min组与推拿30 min组比较差异无统计学意义。结论:推拿治疗CSA家兔需要达到20 min时才可以显著改善椎动脉血流量,但是继续延长推拿时间则无明显效果,推拿这种时效性特点的物质基础与血清中NPY和ET-1水平的变化密切相关。

Experimental study on improvement of blood supply timeliness of rabbits with vertebral artery type of cervical spondylosis by massage

Objective:Establishing a rabbit model of vertebral artery type of cervical spondylosis(CSA) and to observe the characteristics of timeliness in improving the blood flow of vertebral artery by massage,and discusse the material basis of this timeliness based on NPY and ET-1.Methods:Fifty New Zealand healthy and white rabbits,6-month-old,the body mass of (2.0±0.5) kg,with half males and half females,were randomly divided into blank group,model group,three massage groups(including massage for 10 min,20 min,and 30 min group by random number table),10 rabbits in each group. In addition to the blank group,CSA rabbit model was made by injection of sclerosing agent in other groups. The rabbits of massage for 10 min,20 min,and 30 min groups received the massage therapy of corresponding duration,one times a day,continuous 10 days. The blood flow of vertebral artery in each group was detected by PeriFlux5000 laser doppler,and the contents of NPY and ET-1 in serum were detected by ELISA before and after treatment.Results:Changes in blood flow of vertebral artery before and after treatment:there was no significant difference between model group and massage for 10 min group;there was significant difference between model group and massage for 20,30 min groups(P<0.01);there was significant difference between massage for 10 min group and massage for 20,30 min groups(P<0.01);there was no significant difference between massage for 20 min group and massage for 30 min group. Changes of NPY content before and after treatment:there was significant difference between model group and massage for 10 min group (P<0.05);there was significant difference between model group and massage for 20,30 min groups(P<0.01);there was significant difference between massage for 10 min group and massage for 20,30 min groups(P<0.01);there was no significant difference between massage for 20 min group and massage for 30 min group. Changes of ET-1 content before and after treatment:there was no significant difference between model group and massage for 10 min group;there was significant difference between model group and massage for 20,30 min groups(P<0.01);there was significant difference between massage for 10 min group and massage for 20 min,30 groups (P<0.01);there was no significant difference between massage for 20 min group and massage for 30 min group.Conclusion:Massage needed 20 min for rabbits with CSA can only significantly improve the blood flow of vertebral artery. However,prolonging the time of massage has no obvious effect. The material basis of this timeliness characteristic of massage is closely related to the change of NPY and ET-1 levels in serum.