2007年浙江省鼠类感染汉坦病毒监测及病毒分离

目的了解浙江省鼠类自然感染汉坦病毒（HV）情况和病毒型别，为肾综合征出血热（HFRS）防治提供科学依据。方法采集鼠类肺组织和血清标本，用间接免疫荧光试验检测鼠血清IgG抗体，直接免疫荧光试验检测鼠肺Hv抗原。HV抗原阳性鼠肺接种Vero—E6细胞分离病毒，用HV单克隆荧光抗体鉴定分离株的型别。结果共捕获鼠形动物1129只，其中黑线姬鼠为优势种，鼠肺HV抗原阳性率为3．0％，鼠血标本IgG抗体阳性57份，阳性率8．0％。分离到6株汉滩（HTN）型病毒，其中来自黑线姬鼠5株，褐家鼠1株。结论浙江省存在以黑线姬鼠为主要传染源的HFRS疫源地，HV主要流行型别为HTN型。

Surveillance on natural infection of rodents with Hantavirus in Zhejiang province and isolation of Hantavirus

Objective To clarify the natural infection situation of rodents by Hantavirus （HV） and HV strains in Zhejiang province in 2007, and provide science evidence for the prevention and control of hemorrhagic fever with renal syndrome （HFRS）. Methods Lung tissue and serum from rodent were sampled, and IgG antibody from serum was tested by indirect immunofluorscence assay and direct immunofluorscence assay was adopted to test HV antigens from lung tissues. HV was isolated by Vero-E6 cells, and HV strains were identified by Anti-McAb. Results A total of 1129 rodents were captured in 2007. The dominant specie was Apodemus agrarius, and the positive rate of HV antigen in rodent lungs was 3.0%. The IgG antibody of 57 blood samples was positive, and the positive rate was 8.0%. Six strains of hantaan （HTN） virus were isolated, five strains from A.agrarius and one from Rattus norvegicus. Conclusion There are natural foci of HFRS which main infection sources are A.agrarius in Zhejiang province, and HTN strain could be the main prevalence strains of HV.