肝细胞癌p53基因突变初步研究

本文应用快速银染多了矣酶链反应单链构像多态（ＰＣＲ－ＳＳＣＰ）方法检测了３０例肝细胞癌（ＨＣＣ）组织中ｐ５３基因变异情况。结果１３例癌组织中有异常电带，其中２闰于第５外显子，各有３例位于第６和第７外显子，位于第８显子有５例，ｐ５３基因变异的病例乙型肝炎表面抗原均阳性，实验结果表明：本技术能有效检测出基因突变；肝细胞癌中ｐ53基因突变率较高且有多个位点；肝细胞癌ｐ５３基因突变可能与乙型肝炎病毒感染有关

Detection of point mutation of p53 gene by PCR-SSCP in hepatocellular carcinoma tissue

PCR-SSCP method was used to detect the mutation of p53 gene in hepatocellular carcinoma tissues. The results revealed that the mutation was identified in 13 out of 30 cases. Two cases were found in exon 5,5 in exon 8, 3 in exon 6 and 7 respectively. All cases which had mutations were HBsAg positive. These results suggest PCR-SSCP method be effective to detect gene point mutation,there be a relatively high incidence of p53 gene mutation through exon 5 to exon 8 in hepatocellular carcinoma,and the mutations may be related to hepatitis B virus infection.