LPS对骨肉瘤细胞迁移、侵袭及TLR4/HOTAIR通路的影响

目的探讨脂多糖(LPS)对骨肉瘤细胞迁移和侵袭的影响及其潜在的作用机制。方法将人MG-63骨肉瘤细胞随机分为2组:对照组和LPS组。LPS组细胞用10 g/ml的LPS干预24 h,对照组用生理盐水干预。ELISA检测干预后培养基中促炎因子的水平,Transwell实验检测细胞迁移和侵袭能力,Western Blot检测相关蛋白的表达。结果与对照组相比,LPS组培养基中促炎因子TNF-α、IL-1和IL-6的释放水平均显著增高(P<0.05),LPS组迁移细胞数和侵袭细胞数均显著增高(P<0.05),LPS组中E-cadherin的表达显著降低(P<0.05),而N-cadherin、α-SMA、波形蛋白、TLR4和HOTAIR的表达均显著增高(P<0.05)。结论LPS诱导的肿瘤微环境可促进骨肉瘤细胞的迁移和侵袭,其机制与TLR4/HOTAIR途径介导的EMT过程的发生有密切关系。

Effect of LPS on Migration,Invasion and TLR4/HOTAIR Pathway of Osteosarcoma Cells

Objective To investigate the effect and potential mechanism of LPS on the migration and invasion of osteosarcoma cells. Methods Human MG-63 osteosarcoma cells were randomly divided into 2 groups: the control group and the LPS group. The cells in the LPS group were treated with 10 g/ml LPS for 24 h,and the cells in the control group were treated with physiological saline. The levels of pro-inflammatory factors in the medium after intervention were detected by ELISA. The cell migration and invasion ability were detected by Transwell assay,and the expression of related proteins was detected by Western Blot.Results Compared with the control group,the release of pro-inflammatory factors including TNF-,IL-1 and IL-6 in the LPS group were significantly increased( P < 0. 05),and the numbers of migrated cells and the numbers of invasive cells in the LPS group were significantly increased( P < 0. 05),the expression of E-cadherin was significantly decreased in the LPS group( P < 0. 05),while the expression of N-cadherin,-SMA,vimentin,TLR4 and HOTAIR were significantly increased( P < 0. 05). Conclusion LPS-induced tumor microenvironment can promote the migration and invasion of osteosarcoma cells,and its mechanism is closely related to the occurrence of EMT process mediated by TLR4/HOTAIR pathway.